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Direct-Blot™ HRP anti-human IgG1 Fc Antibody

Pricing & Availability
Clone
M1308A10 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin Heavy Constant Gamma 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M1308A10_DB-HRP_IgG_Antibody_062017
Mouse serum (lane 1)and human serum (lane 2) (10µg protein for each) were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1:1000 purified anti-human IgG Fc Antibody or 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti-human IgG1 Fc Antibody (upper). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP. Ponceau S staining (lower) was used as loading control. Lane M is the Molecular weight ladder.
  • M1308A10_DB-HRP_IgG_Antibody_062017
    Mouse serum (lane 1)and human serum (lane 2) (10µg protein for each) were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1:1000 purified anti-human IgG Fc Antibody or 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti-human IgG1 Fc Antibody (upper). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP. Ponceau S staining (lower) was used as loading control. Lane M is the Molecular weight ladder.
  • M1308A10_DB-HRP_IgG_Antibody_2_061518
    Human serum (lane 1) and mouse serum (lane 2) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:1000 Direct-Blot™ HRP anti-human IgG1 Fc Antibody (upper). Proteins were visualized by chemiluminescence detection. Ponceau S staining (lower) was used as loading control. Lane M is the Molecular weight ladder.
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410604 25 µL 101 CHF
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410603 100 µL 165 CHF
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Description

IgG Fc is a homodimer that is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through the binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human IgG Fc fusion protein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:10,000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Additional Product Notes

This product was previously named anti-human IgG Fc Antibody.

Product Citations
  1. Fiškin E, et al. 2022. Nat Biotechnol. 40:374. PubMed
  2. Thirugnanam K, et al. 2023. Front Mol Biosci. 1250016:10. PubMed
  3. Powell AE, et al. 2020. bioRxiv. . PubMed
  4. Powell AE, et al. 2021. ACS Cent Sci. 7:183. PubMed
RRID
AB_2687208 (BioLegend Cat. No. 410604)
AB_2687208 (BioLegend Cat. No. 410603)

Antigen Details

Structure
Predicted molecular weight of approximately 50 kD.
Function
Opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation.
Ligand/Receptor
CD16, CD32, and CD64.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology
Antigen References

1. Paul, WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia.

Gene ID
3500 View all products for this Gene ID
UniProt
View information about IgG1 Fc on UniProt.org
Go To Top Version: 3    Revision Date: 08.29.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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