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Purified anti-human IgG1 Fc Antibody

Pricing & Availability
Clone
M1308A10 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin Heavy Constant Gamma 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M1308A10_PURE_IgG_Fc_Antibody_WB_082018
Total protein lysates (15 µg protein) from human serum (lane 1), mouse serum (lane 2), human IgG1 recombinant antibody (lane 3) and human IgG2 recombinant antibody (lane 4) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-human IgG1 Fc antibody, clone M1308A10 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using a 1:3000 diluted anti-rat-IgG secondary antibody conjugated to HRP for purified anti-human IgG Fc antibody. Ponceau S staining (lower panel) was used as loading control for human and mouse serum. Lane M: Molecular weight ladder.
  • M1308A10_PURE_IgG_Fc_Antibody_WB_082018
    Total protein lysates (15 µg protein) from human serum (lane 1), mouse serum (lane 2), human IgG1 recombinant antibody (lane 3) and human IgG2 recombinant antibody (lane 4) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-human IgG1 Fc antibody, clone M1308A10 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using a 1:3000 diluted anti-rat-IgG secondary antibody conjugated to HRP for purified anti-human IgG Fc antibody. Ponceau S staining (lower panel) was used as loading control for human and mouse serum. Lane M: Molecular weight ladder.
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410601 100 µg 112 CHF
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Description

IgG Fc is a homodimer that is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC) and complement activation through the binding to Fc receptors such as CD16, CD32, CD64 and the complement factor C1. Clone M1308A10 was generated by immunogen derived from recombinant human IgG1 Fc fusion protein and only recognizes human IgG1 Fc heavy chain, but not human IgG2 Fc heavy chain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone M1308A10 was generated by immunogen derived from recombinant human IgG1 Fc fusion protein and only recognizes human IgG1 Fc heavy chain, but not human IgG2 Fc heavy chain.

Additional Product Notes

This product was previously named anti-human IgG Fc Antibody.

Product Citations
  1. Fox CR, et al. 2021. Viruses. 14:. PubMed
RRID
AB_2565267 (BioLegend Cat. No. 410601)

Antigen Details

Structure
Predicted molecular weight of approximately 50 kD.
Function
Opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation.
Ligand/Receptor
CD16, CD32, and CD64.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology
Antigen References

1. Paul, WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia.

Gene ID
3500 View all products for this Gene ID
UniProt
View information about IgG1 Fc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 04.24.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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