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Direct-Blot™ HRP anti-IRF8 Antibody

Pricing & Availability
Clone
7G11A45 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 8, Interferon consensus sequence-binding protein (ICSBP)
Isotype
Mouse IgG1, κ
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Product Citations
publications
7G11A45_DBHRP_IRF8_Antibody_WB_062716
15 µg of total protein extract from THP-1 was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP anti-IRF8 antibody (clone 7G11A45). Proteins were visualized using chemiluminescence detection. Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control.
  • 7G11A45_DBHRP_IRF8_Antibody_WB_062716
    15 µg of total protein extract from THP-1 was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP anti-IRF8 antibody (clone 7G11A45). Proteins were visualized using chemiluminescence detection. Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control.
  • 7G11A45_DBHRP_IRF8_Antibody_WB_2_070218
    Total cell lysates (15 µg protein) from THP-1 (lane 1) and HeLa (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:4000 diluted Direct-Blot™ HRP anti-IRF8 Antibody, clone 7G11A45 (upper), or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1 (lower). Proteins were visualized by chemiluminescence detection. Lane M: Molecular weight ladder.
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656503 100 µL 340 CHF
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Description

IRF8 is a member of the IRF family of transcription factors, playing important roles in IFN signaling pathways and innate immune responses. It regulates hematopoietic cell growth and differentiation of macrophage and dendritic cells. Homozygous IRF8-deficient (IRF8-/-) mice have impaired production of interferon and IL-12, exhibit increased susceptibility to viral pathogens, and have marked expansion of granulocytes that results in a phenotype similar to chronic myelogenous leukemia (CML). IRF8 is also expressed in B lymphocytes, involved in differentiation and commitment of B cell lineage. The DNA-binding activity of IRF8 is significantly increased when interacting with other transcription factors, such as the other members of the IRF family and the ETS family transcription factors. Therefore, the transcriptional activity of IRF8 is dependent on the transcription factors with which it associates.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF8 recombinant protein (116-220 a.a.)
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

RRID
AB_2617029 (BioLegend Cat. No. 656503)

Antigen Details

Structure
426 amino acids, predicted molecular weight of 48 kD, contains a IRF tryptophan pentad repeat responsible for DNA binding and a C-terminal IRF association domain (IAD) responsible for heterodimerization with other transcription factors
Distribution

Nucleus

Function
Transcription factor primarily expressed in hematopoietic cells, regulates expression of type I IFN and IFN-inducible genes in response to viral infection
Interaction
Interacts with BATF, JunB, TRIM21, and COPS2
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Minten C, et al. 2012. PLoS One. 7:e49851.
2. Xu H, et al. 2012. Nat. Immunol. 13:642.
3. Ouyang X, et al. 2011. Nat. Commun. 2:314.
4. Shin DM, et al. 2011. PLoS One. 6:e27384.
5. Wang H, et al. 2009. Immunol. Res. 43:109.
6. Qi CF, et al. 2009. Immunol. Res. 45:62.

Gene ID
3394 View all products for this Gene ID
UniProt
View information about IRF8 on UniProt.org
Go To Top Version: 1    Revision Date: 06.27.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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