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Purified anti-IRF8 Antibody

Pricing & Availability
Clone
7G11A45 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 8, Interferon consensus sequence-binding protein (ICSBP)
Isotype
Mouse IgG1, κ
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Product Citations
publications
7G11A45_PURE_IRF8_Antibody_1_WB_070218
Total cell lysates (15 µg protein) from THP-1 (lane 1) and HeLa (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL Purified anti-IRF 8 Antibody (1:1000 dilution), clone 7G11A45 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-IRF 8 Antibody, or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder.
  • 7G11A45_PURE_IRF8_Antibody_1_WB_070218
    Total cell lysates (15 µg protein) from THP-1 (lane 1) and HeLa (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL Purified anti-IRF 8 Antibody (1:1000 dilution), clone 7G11A45 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-IRF 8 Antibody, or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder.
  • 7G11A45_PURE_IRF8_Antibody_2_IF_070218
    THP-1 cells were stained with purified anti-IRF8 (7G11A45) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
  • 7G11A45_PURE_IRF8_Antibody_3_IP_070218
    Immunoprecipitation of IRF8 from THP-1 cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-IRF8 antibody (clone 7G11A45, lane 4). Western blot was performed using anti-IRF8 antibody (clone 7G11A45).
Cat # Size Price Quantity Check Availability Save
656501 25 µg 112 CHF
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656502 100 µg 265 CHF
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Description

IRF8 is a member of the IRF family of transcription factors, playing important roles in IFN signaling pathways and innate immune responses. It regulates hematopoietic cell growth and differentiation of macrophage and dendritic cells. Homozygous IRF8-deficient (IRF8-/-) mice have impaired production of interferon and IL-12, exhibit increased susceptibility to viral pathogens, and have marked expansion of granulocytes that results in a phenotype similar to chronic myelogenous leukemia (CML). IRF8 is also expressed in B lymphocytes, involved in differentiation and commitment of B cell lineage. The DNA-binding activity of IRF8 is significantly increased when interacting with other transcription factors, such as the other members of the IRF family and the ETS family transcription factors. Therefore, the transcriptional activity of IRF8 is dependent on the transcription factors with which it associates.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF8 recombinant protein (116-220 a.a.)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  2. Schwabenland M, et al. 2021. Immunity. . PubMed
  3. McDaniel MM, et al. 2020. Cell Reports. 31(5):107604. PubMed
RRID
AB_2562395 (BioLegend Cat. No. 656501)
AB_2562395 (BioLegend Cat. No. 656502)

Antigen Details

Structure
426 amino acids, predicted molecular weight of 48 kD, contains a IRF tryptophan pentad repeat responsible for DNA binding and a C-terminal IRF association domain (IAD) responsible for heterodimerization with other transcription factors
Distribution

Nucleus

Function
Transcription factor primarily expressed in hematopoietic cells, regulates expression of type I IFN and IFN-inducible genes in response to viral infection
Interaction
Interacts with BATF, JunB, TRIM21, and COPS2
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Minten C, et al. 2012. PLoS One. 7:e49851.
2. Xu H, et al. 2012. Nat. Immunol. 13:642.
3. Ouyang X, et al. 2011. Nat. Commun. 2:314.
4. Shin DM, et al. 2011. PLoS One. 6:e27384.
5. Wang H, et al. 2009. Immunol. Res. 43:109.
6. Qi CF, et al. 2009. Immunol. Res. 45:62.

Gene ID
3394 View all products for this Gene ID
UniProt
View information about IRF8 on UniProt.org
Go To Top Version: 3    Revision Date: 07.02.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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