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Direct-Blot™ HRP anti-mCherry Antibody

Pricing & Availability
Clone
8C5.5 (See other available formats)
Regulatory Status
RUO
Other Names
DSRED
Isotype
Mouse IgG2a, κ
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Product Citations
publications
85C.5_mCherry_WB_Image_010616
10 µg of total protein extract from 293E cells transfected with pcDNA3.1-mCherry was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-mCherry (clone 8C5.5). Proteins were visualized using chemiluminescence detection.
  • 85C.5_mCherry_WB_Image_010616
    10 µg of total protein extract from 293E cells transfected with pcDNA3.1-mCherry was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-mCherry (clone 8C5.5). Proteins were visualized using chemiluminescence detection.
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677703 100 µL 340 CHF
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Description

mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins1,2. mCherry derives from proteins initially isolated from Cnidarians.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
mCherry.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Product Citations
  1. Irie F, et al. 2021. J Biol Chem. 296:100481. PubMed
RRID
AB_2566703 (BioLegend Cat. No. 677703)

Antigen Details

Structure
236 amino acids with a predicted molecular weight of approximately 27 kD.
Function
Fluorescent protein.
Biology Area
Cell Biology
Antigen References

1. Shaner NC, et al. 2008. Nat. Methods 5:545.
2. Shaner NC, et al. 2005. Nat. Methods 2:905.

Gene ID
NA
UniProt
View information about mCherry on UniProt.org
Go To Top Version: 4    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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