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Purified anti-mCherry Antibody

Pricing & Availability
Clone
8C5.5 (See other available formats)
Regulatory Status
RUO
Other Names
DSRED
Isotype
Mouse IgG2a, κ
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Product Citations
publications
1_8C5.5_PURE_mCherry_Antibody_1_WB_070115
Total cell lysates (15 µg total protein) from mock-transfected 293E or 293E transfected with mCherry- fused protein were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) of Purified anti-mCherry Antibody, clone 8C5.5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:1000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_8C5.5_PURE_mCherry_Antibody_1_WB_070115
    Total cell lysates (15 µg total protein) from mock-transfected 293E or 293E transfected with mCherry- fused protein were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) of Purified anti-mCherry Antibody, clone 8C5.5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:1000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_8C5.5_PURE_mCherry_Antibody_2_IF_070115
    mCherry-transfected 293T cells were stained with purified anti-mCherry (clone 8C5.5) antibody, followed by staining with DyLight™ 488 Goat anti-mouse IgG antibody (green). Nuclei were counterstained with DAPI (blue). Non-transfected 293T cells showed nuclei staining only. The image was captured with a 40X objective.
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677701 25 µg 124 CHF
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677702 100 µg 311 CHF
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Description

mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins1,2. mCherry derives from proteins initially isolated from Cnidarians.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
mCherry.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg per ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Yoo TY, et al. 2021. J Cell Biol. 220:. PubMed
  2. Fote GM, et al. 2022. J Cell Sci. 135:. PubMed
  3. Cottier S, et al. 2021. J Cell Sci. :. PubMed
RRID
AB_2565910 (BioLegend Cat. No. 677701)
AB_2565910 (BioLegend Cat. No. 677702)

Antigen Details

Structure
236 amino acids with a predicted molecular weight of approximately 27 kD.
Function
Fluorescent protein.
Biology Area
Cell Biology
Antigen References

1. Shaner NC, et al. 2008. Nat. Methods 5:545.
2. Shaner NC, et al. 2005. Nat. Methods 2:905.

Gene ID
NA
UniProt
View information about mCherry on UniProt.org
Go To Top Version: 3    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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