Loading...

FITC anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
M5E2_FITC_100507
Human peripheral blood monocytes stained with M5E2 FITC
  • M5E2_FITC_100507
    Human peripheral blood monocytes stained with M5E2 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
301803 25 tests 49 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
301804 100 tests 116 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Zhao M, et al. 2021. Frontiers in Cell and Developmental Biology. 9:652972. PubMed
  2. Wang H, et al. 2020. Cell Stem Cell. 28(3):535-549.e8. PubMed
  3. Aarsund M, et al. 2022. Cancer Immunol Immunother. 71:2227. PubMed
  4. Abe I, et al. 2022. Dev Cell. 57:2623. PubMed
  5. Matsumoto H, et al. 2023. Front Immunol. 13:978435. PubMed
  6. Aabling RR, et al. 2023. Regen Ther. 23:67. PubMed
  7. Gao Y, et al. 2023. iScience. 26:106729. PubMed
  8. Shen WY, et al. 2023. iScience. 26:106805. PubMed
  9. Jiang D, et al. 2023. Nat Commun. 14:3642. PubMed
  10. Wang Y, et al. 2023. Mol Med Rep. 28:. PubMed
  11. Chen X, et al. 2022. Front Bioeng Biotechnol. 10:915181. PubMed
  12. Chiu YL, et al. 2018. Immun Ageing. 15:27. PubMed
  13. Shoda H, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1308-y. PubMed
  14. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  15. Poran A, et al. 2020. Cell Reports Medicine. 1(8):100141. PubMed
  16. Zhao Y, et al. 2021. Front Immunol. 12:665442. PubMed
  17. Bencze D, et al. 2022. Int J Mol Sci. 23:. PubMed
  18. Wiernik A, et al. 2013. Clin Cancer Res. 19:3844. PubMed
  19. Schulthess J et al. 2019. Immunity. 50(2):432-445 . PubMed
  20. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  21. Hecker A, et al. 2015. J Immunol. 195: 2325-2334. PubMed
  22. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  23. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  24. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  25. Ma X, et al. 2022. Front Oncol. 12:855570. PubMed
  26. Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
  27. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  28. Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
  29. Forbester JL, et al. 2020. J Virol. 94:. PubMed
  30. Jankeel A, et al. 2020. J Virol. :94. PubMed
  31. Qiu XM, et al. 2020. Reproduction. 251:159. PubMed
  32. Li F, et al. 2022. Front Pharmacol. 13:1001552. PubMed
  33. Grivas A, et al. 2022. Front Immunol. 13:964274. PubMed
  34. Qi F, et al. 2022. Oncol Lett. 24:273. PubMed
  35. Saha R, et al. 2021. Sci Rep. 11:18849. PubMed
  36. Wang D, et al. 2020. J Cell Mol Med. 24:4212. PubMed
  37. Crifò B, et al. 2019. J Immunol. 202:1521. PubMed
  38. Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed
  39. Zhang S, et al. 2021. Stem Cells Int. 6616240:2021. PubMed
  40. Yao J, et al. 2022. Front Pharmacol. 13:876076. PubMed
  41. Cheng Y, et al. 2021. Immunity. 54(8):1825-1840.e7. PubMed
  42. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  43. Pucino V, et al. 2020. Cell Metabolism. 30(6):1055-1074.e8.. PubMed
  44. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  45. Autenrieth S, et al. 2015. Clin Transl Immunology. 4:50. PubMed
  46. Jackson R, et al. 2020. Viruses. 12:00. PubMed
  47. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  48. Donini M, et al. 2011. Prostate. 72:566. PubMed
  49. Rao Z, et al. 2019. J Immunol. 203:1031. PubMed
  50. Kletting S, et al. 2017. ALTEX. 35:211. PubMed
  51. Yang X, et al. 2019. Mol Med Rep. 19:1083. PubMed
  52. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  53. Lin F, et al. 2020. Int J Oncol. 56:151. PubMed
  54. Zheng H, et al. 2022. Cell Rep. 39:110864. PubMed
  55. Liao HQ, et al. 2021. Am J Reprod Immunol. 86:e13406. PubMed
  56. Wu Q, et al. 2022. Cancer Immunol Res. 10:182. PubMed
  57. Wang Z, et al. 2020. J Cell Mol Med. . PubMed
  58. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
  59. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  60. Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed
  61. Fekete T, et al. 2020. Front Immunol. 11:572960. PubMed
  62. Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed
  63. Wells DK, et al. 2020. Cell. 183:818. PubMed
  64. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  65. Zavareh RB, et al. 2020. Cell Chemical Biology. 28(2):158-168.e5. PubMed
  66. Pierini S, et al. 2020. JCI Insight. 5:00. PubMed
  67. Qu M, et al. 2021. Frontiers in Oncology. 11:659720. PubMed
  68. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  69. Hiatt J, et al. 2021. Cell Reports. 35(6):109105. PubMed
  70. Wang SQ, et al. 2021. J Viral Hepat. 28:795. PubMed
  71. Langnau C, et al. 2021. Arterioscler Thromb Vasc Biol. 41:2080. PubMed
  72. Meng Q, et al. 2022. Oncol Lett. 23:83. PubMed
  73. Ceglia V, et al. 2022. Front Immunol. 12:786144. PubMed
  74. Ren X, et al. 2021. Cell Death Dis. 12:484. PubMed
RRID
AB_314185 (BioLegend Cat. No. 301803)
AB_314185 (BioLegend Cat. No. 301804)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Other Formats

View All CD14 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD14 M5E2 FC
FITC anti-human CD14 M5E2 FC
PE anti-human CD14 M5E2 FC
Purified anti-human CD14 M5E2 FC,CyTOF®,Block,IHC-F
PE/Cyanine7 anti-human CD14 M5E2 FC
Alexa Fluor® 488 anti-human CD14 M5E2 FC
Alexa Fluor® 647 anti-human CD14 M5E2 FC
Ultra-LEAF™ Purified anti-human CD14 M5E2 FC,CyTOF®,Block,IHC-F
Pacific Blue™ anti-human CD14 M5E2 FC
APC/Cyanine7 anti-human CD14 M5E2 FC
Alexa Fluor® 700 anti-human CD14 M5E2 FC
PerCP/Cyanine5.5 anti-human CD14 M5E2 FC
Biotin anti-human CD14 M5E2 FC
Brilliant Violet 421™ anti-human CD14 M5E2 FC
Brilliant Violet 570™ anti-human CD14 M5E2 FC
Brilliant Violet 605™ anti-human CD14 M5E2 FC
Brilliant Violet 650™ anti-human CD14 M5E2 FC
Brilliant Violet 711™ anti-human CD14 M5E2 FC
Brilliant Violet 785™ anti-human CD14 M5E2 FC
Brilliant Violet 510™ anti-human CD14 M5E2 FC
Purified anti-human CD14 (Maxpar® Ready) M5E2 FC,CyTOF®
PerCP anti-human CD14 M5E2 FC
FITC anti-human CD14 M5E2 FC
PE/Dazzle™ 594 anti-human CD14 M5E2 FC
Pacific Blue™ anti-human CD14 M5E2 FC
APC/Fire™ 750 anti-human CD14 M5E2 FC
APC anti-human CD14 M5E2 FC
TotalSeq™-A0081 anti-human CD14 M5E2 PG
TotalSeq™-B0081 anti-human CD14 M5E2 PG
TotalSeq™-C0081 anti-human CD14 M5E2 PG
PE anti-human CD14 M5E2 FC
PE/Cyanine5 anti-human CD14 M5E2 FC
TotalSeq™-D0081 anti-human CD14 M5E2 PG
APC/Fire™ 750 anti-human CD14 M5E2 FC
GMP FITC anti-human CD14 M5E2 FC
PE/Cyanine7 anti-human CD14 M5E2 FC
GMP APC anti-human CD14 M5E2 FC
GMP PE anti-human CD14 M5E2 FC
PE/Dazzle™ 594 anti-human CD14 M5E2 FC
GMP Pacific Blue™ anti-human CD14 M5E2 FC
GMP APC/Fire™ 750 anti-human CD14 M5E2 FC
PerCP/Cyanine5.5 anti-human CD14 M5E2 FC
Spark Violet™ 500 anti-human CD14 M5E2 FC
GMP PE/Dazzle™ 594 anti-human CD14 M5E2 FC
APC/Fire™ 810 anti-human CD14 M5E2 FC
PE/Fire™ 700 anti-human CD14 M5E2 FC
Go To Top Version: 4    Revision Date: 11.05.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account