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Purified anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
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Product Citations
publications
M5E2
Human peripheral blood monocytes stained with M5E2 APC
  • M5E2
    Human peripheral blood monocytes stained with M5E2 APC
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301801 25 µg 42 CHF
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301802 100 µg 90 CHF
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Block, IHC-F, PG - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Zizzo G, et al. 2012. J Immunol. 189:3508. PubMed
  2. Humblet–Baron S, et al. 2019. J Allergy Clin Immunol. 143:2215. PubMed
  3. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  4. McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed
  5. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  6. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  7. Feyaerts D, et al. 2022. Cell Rep Med. 3:100680. PubMed
  8. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  9. Kaushal A, et al. 2021. Blood Cancer Discov. 2:600. PubMed
  10. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  11. Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed
  12. Nguyen LT, et al. 2019. Cancer Immunol Immunother. 68:773. PubMed
  13. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  14. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  15. Gao H, et al. 2021. J Virol. :. PubMed
  16. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  17. Tal MC, et al. 2020. MBio. 11:00. PubMed
  18. Winkler M, et al. 2017. PLoS One.. 10.1371/journal.pone.0182427. PubMed
  19. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  20. Coelho CH, et al. 2020. JCI Insight. 5:. PubMed
  21. Raffray L, et al. 2020. Immunology. 159:88. PubMed
  22. Li H, et al. 2020. Front Immunol. 11:1777. PubMed
  23. Kong R, et al. 2019. Cell. 178:567. PubMed
  24. Olin A, et al. 2018. Cell. 174:1277. PubMed
  25. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  26. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  27. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  28. Auladell M, et al. 2019. Clin Transl Immunology. 8:e01090. PubMed
  29. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  30. Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
  31. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  32. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  33. Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
  34. Henrick BM, et al. 2021. Cell. . PubMed
  35. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  36. Schaut RG, et al. 2019. Sci Rep. 4.820138889. PubMed
  37. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  38. Schwabenland M, et al. 2021. Immunity. . PubMed
  39. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  40. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
  41. Oda H, et al. 2019. Front Immunol. 10:479. PubMed
  42. Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
  43. Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
  44. Iwamoto S, et al. 2007. J Immunol. 179:1449. PubMed
  45. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  46. Sung JA, et al. 2018. Mol Ther. 26:2496. PubMed
  47. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  48. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
  49. Martin E, et al. 2020. JCI Insight. :5. PubMed
  50. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  51. Roberts JT, et al. 2020. Mol Cell Proteomics. 19:362. PubMed
  52. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
RRID
AB_314183 (BioLegend Cat. No. 301801)
AB_314183 (BioLegend Cat. No. 301802)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Other Formats

View All CD14 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD14 M5E2 FC
FITC anti-human CD14 M5E2 FC
PE anti-human CD14 M5E2 FC
Purified anti-human CD14 M5E2 FC,CyTOF®,Block,IHC-F
PE/Cyanine7 anti-human CD14 M5E2 FC
Alexa Fluor® 488 anti-human CD14 M5E2 FC
Alexa Fluor® 647 anti-human CD14 M5E2 FC
Ultra-LEAF™ Purified anti-human CD14 M5E2 FC,CyTOF®,Block,IHC-F
Pacific Blue™ anti-human CD14 M5E2 FC
APC/Cyanine7 anti-human CD14 M5E2 FC
Alexa Fluor® 700 anti-human CD14 M5E2 FC
PerCP/Cyanine5.5 anti-human CD14 M5E2 FC
Biotin anti-human CD14 M5E2 FC
Brilliant Violet 421™ anti-human CD14 M5E2 FC
Brilliant Violet 570™ anti-human CD14 M5E2 FC
Brilliant Violet 605™ anti-human CD14 M5E2 FC
Brilliant Violet 650™ anti-human CD14 M5E2 FC
Brilliant Violet 711™ anti-human CD14 M5E2 FC
Brilliant Violet 785™ anti-human CD14 M5E2 FC
Brilliant Violet 510™ anti-human CD14 M5E2 FC
Purified anti-human CD14 (Maxpar® Ready) M5E2 FC,CyTOF®
PerCP anti-human CD14 M5E2 FC
FITC anti-human CD14 M5E2 FC
PE/Dazzle™ 594 anti-human CD14 M5E2 FC
Pacific Blue™ anti-human CD14 M5E2 FC
APC/Fire™ 750 anti-human CD14 M5E2 FC
APC anti-human CD14 M5E2 FC
TotalSeq™-A0081 anti-human CD14 M5E2 PG
TotalSeq™-B0081 anti-human CD14 M5E2 PG
TotalSeq™-C0081 anti-human CD14 M5E2 PG
PE anti-human CD14 M5E2 FC
PE/Cyanine5 anti-human CD14 M5E2 FC
TotalSeq™-D0081 anti-human CD14 M5E2 PG
APC/Fire™ 750 anti-human CD14 M5E2 FC
GMP FITC anti-human CD14 M5E2 FC
PE/Cyanine7 anti-human CD14 M5E2 FC
GMP APC anti-human CD14 M5E2 FC
GMP PE anti-human CD14 M5E2 FC
PE/Dazzle™ 594 anti-human CD14 M5E2 FC
GMP Pacific Blue™ anti-human CD14 M5E2 FC
GMP APC/Fire™ 750 anti-human CD14 M5E2 FC
PerCP/Cyanine5.5 anti-human CD14 M5E2 FC
Spark Violet™ 500 anti-human CD14 M5E2 FC
GMP PE/Dazzle™ 594 anti-human CD14 M5E2 FC
APC/Fire™ 810 anti-human CD14 M5E2 FC
PE/Fire™ 700 anti-human CD14 M5E2 FC
Go To Top Version: 5    Revision Date: 05.14.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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