FITC anti-mouse CD326 (Ep-CAM) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

TE-71 thymic epithelial cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed¹, with or without OCT embedding^2,4, and spatial biology (IBEX)^13,14.

Application References

(PubMed link indicates BioLegend citation)

1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
7. Ishii K, et al. 2012. Development. 139:1734. PubMed
8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

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4. Miyao T, et al. 2022. Elife. 11: . PubMed
5. Pribluda A, et al. 2022. Elife. 11: . PubMed
6. Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed
7. Kobayashi S, et al. 2022. Inflamm Regen. 42:49. PubMed
8. Ishikawa T, et al. 2023. Bio Protoc. 13:e4588. PubMed
9. Papa FR, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L564. PubMed
10. Korsunsky I, et al. 2022. Med. 3:481. PubMed
11. Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed
12. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
13. Padonou F, et al. 2022. EMBO Rep. 23:e53576. PubMed
14. Kurosawa M, et al. 2021. Int J Mol Sci. 22:. PubMed
15. Saenz F, et al. 2014. PLoS One. 9:97666. PubMed
16. Jard T, et al. 2020. Cell Stem Cell. 27(4):646-662.e7. PubMed
17. Bittner-Eddy P, et al. 2016. J Immunol. 197: 1435 - 1446. PubMed
18. Cleary A, et al. 2014. Nature. 508:113. PubMed
19. Helm M, et al. 2022. Life (Basel). 12:. PubMed
20. Atapattu L, et al. 2016. J Exp Med. 213: 1741 - 1757. PubMed
21. Wlodarska M et al. 2017. Cell host & microbe. 22(1):25-37 . PubMed
22. Panciera T, et al. 2020. Nat Mater. 1.345138889. PubMed
23. Emgård J, et al. 2018. Immunity. 143:419. PubMed
24. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
25. Furstenberg R, et al. 2011. Am J Physiol Gastrointest Liver Physiol. 300:G409. PubMed
26. Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed
27. Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
28. Yamamoto M, et al. 2019. Commun Biol. 2:292. PubMed
29. Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
30. Watson JK, et al. 2020. Sci Rep. 10:10490. PubMed
31. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
32. Kim SM, et al. 2019. FASEB J. 33:13386. PubMed
33. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
34. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
35. Pervolarakis N, et al. 2020. Cell Rep. 108273:33. PubMed
36. Kamata T, et al. 2020. Cell Reports. 31(12):107802. PubMed
37. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
38. Shibata T, et al. 2020. J Clin Invest. 130:3021. PubMed
39. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
40. King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
41. Matern M, et al. 2017. Sci Rep. 7:42079. PubMed
42. Sato Y, et al. 2018. JCI Insight. 3:. PubMed
43. DiMarco AV, et al. 2022. Cancer Immunol Res. 10:70. PubMed
44. Zhao Q, et al. 2020. Cancer Res. 80:5531. PubMed
45. Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
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47. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed

RRID

AB_1134106 (BioLegend Cat. No. 118207)
AB_1134106 (BioLegend Cat. No. 118208)

Antigen Details

Structure

40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.

Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function

Mediates calcium-independent homophilic cell-cell adhesion.

Interaction

CD326 displays hemophilic binding.

Ligand/Receptor

CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.

Cell Type

Embryonic Stem Cells, Epithelial cells

Biology Area

Immunology, Stem Cells

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID

17075 View all products for this Gene ID

UniProt

View information about CD326 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis