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Purified anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_Pure_060308
TE-71 cell line stained with G8.8 purified, followed by anti-rat IgG PE
  • G8dot8_Pure_060308
    TE-71 cell line stained with G8.8 purified, followed by anti-rat IgG PE
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118201 50 µg 71 CHF
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118202 500 µg 282 CHF
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  2. Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
  3. Thorsen AS, et al. 2020. Dis Model Mech. 14:dmm046706. PubMed
  4. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  5. Tremblay M, et al. 2020. Elife. 9: . PubMed
  6. Nozaki K, et al. 2022. Nature. 606:960. PubMed
  7. Huang L, et al. 2020. JCI Insight. 5:00. PubMed
  8. Nudel I, et al. 2011. J Immunol. 186:891. PubMed
  9. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  10. Casey AE, et al. 2018. J Cell Biol. 217:2951. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:547. PubMed
  12. Karabacak NM, et al. 2022. Cancer Res. 82:1084. PubMed
  13. Ishii K, et al. 2012. Development. 139:1734. PubMed
  14. Aparicio-Domingo P, et al. 2015. J Exp Med. 101084/jm20150318. PubMed
  15. Foster DS, et al. 2020. Nat Commun. 3.278472222. PubMed
  16. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  17. Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
  18. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  19. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  20. Fuhrmann L, et al. 2021. Int J Mol Sci. 22:. PubMed
  21. E-Lacerda RR, et al. 2020. Clin Exp Allergy. 256:50. PubMed
  22. Corcos N, et al. 2021. Frontiers in Immunology. 12:616215. PubMed
  23. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  24. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  25. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  26. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  27. Billings SE, et al. 2021. Development. 148: . PubMed
  28. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  29. Thorsen AS, et al. 2020. Dis Model Mech. . PubMed
  30. Tomchaney M, et al. 2021. BMC Pulm Med. 21:275. PubMed
  31. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  32. Yamamoto K, et al. 2022. STAR Protoc. 3:101155. PubMed
  33. Yamamoto K, et al. 2021. iScience. 24:103064. PubMed
  34. Molina LM, et al. 2021. Cell Reports. 36(1):109310. PubMed
  35. Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed
  36. Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed
  37. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  38. Van Winkle JA, et al. 2020. J Virol. 94:. PubMed
  39. Iyer N, et al. 2020. PLoS Pathog. 16:e1008360. PubMed
  40. Zhou Y, et al. 2020. Am J Pathol. 190:1414. PubMed
  41. Wu W, et al. 2017. Sci Rep. 7:44481. PubMed
RRID
AB_1089026 (BioLegend Cat. No. 118201)
AB_1089026 (BioLegend Cat. No. 118202)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Other Formats

View All CD326 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,ICC
Biotin anti-mouse CD326 (Ep-CAM) G8.8 FC
PE anti-mouse CD326 (Ep-CAM) G8.8 FC
FITC anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC,SB
PE/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
APC/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM) G8.8 ICC,IHC-F,3D IHC
Brilliant Violet 421™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 605™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) (Maxpar® Ready) G8.8 FC,CyTOF®
APC/Fire™ 750 anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 711™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 510™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Dazzle™ 594 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-A0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Alexa Fluor® 700 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-C0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 785™ anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-B0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 650™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Cyanine5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Spark Red™ 718 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 574 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 550 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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