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Pacific Blue™ anti-mouse CD106 Antibody

Pricing & Availability
Clone
429 (MVCAM.A) (See other available formats)
Regulatory Status
RUO
Other Names
VCAM-1, INCAM-110
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_429_PB_100609
C57BL/6 bone marrow cells stained with 429 Pacific Blue™ and CD11b (M1/70) PE
  • A_429_PB_100609
    C57BL/6 bone marrow cells stained with 429 Pacific Blue™ and CD11b (M1/70) PE
  • B_429_PB_100609
    C57BL/6 bone marrow cells stained with rat IgG2a isotype control Pacific Blue™ and CD11b (M1/70) PE
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
105722 100 µg 293 CHF
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Description

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse preadipose cell line PA6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, immunoprecipitation1 , and spacial biology (IBEX)11,12. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Application References

(PubMed link indicates BioLegend citation)
  1. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
  2. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  3. Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
  4. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  5. Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
  6. Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
  7. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  8. Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
  9. Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
  10. Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
  11. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  12. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  2. Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed
RRID
AB_2304290 (BioLegend Cat. No. 105722)

Antigen Details

Structure
Ig superfamily, 47 kD
Distribution

Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells

Function
Adhesion
Ligand/Receptor
VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
Cell Type
Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.

Gene ID
22329 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org

Other Formats

View All CD106 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD106 429 (MVCAM.A) FC
FITC anti-mouse CD106 429 (MVCAM.A) FC
LEAF™ Purified anti-mouse CD106 429 (MVCAM.A) FC, IHC-F, IP
Purified anti-mouse CD106 429 (MVCAM.A) FC,IHC-F,IP
Alexa Fluor® 488 anti-mouse CD106 429 (MVCAM.A) FC,IHC-F,SB
Alexa Fluor® 647 anti-mouse CD106 429 (MVCAM.A) FC,IHC-F,3D IHC
PE anti-mouse CD106 429 (MVCAM.A) FC
PerCP/Cyanine5.5 anti-mouse CD106 429 (MVCAM.A) FC
APC anti-mouse CD106 429 (MVCAM.A) FC
PE/Cyanine7 anti-mouse CD106 429 (MVCAM.A) FC
Pacific Blue™ anti-mouse CD106 429 (MVCAM.A) FC
Alexa Fluor® 594 anti-mouse CD106 429 (MVCAM.A) IHC-F
TotalSeq™-A0226 anti-mouse CD106 429 (MVCAM.A) PG
Ultra-LEAF™ Purified anti-mouse CD106 429 (MVCAM.A) FC,IHC-F,IP
TotalSeq™-C0226 anti-mouse CD106 429 (MVCAM.A) PG
TotalSeq™-B0226 anti-mouse CD106 429 (MVCAM.A) PG
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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