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PE anti-CD247 (TCRζ, CD3ζ) Antibody

Pricing & Availability
Clone
6B10.2 (See other available formats)
Regulatory Status
RUO
Other Names
CD247, CD3zeta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6B10dot2_PE_CD247_Antibody_ICFC_1_052115
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with Fixation Buffer and the Permeabilization buffer. Cells were then stained with CD247 (clone 6B10.2) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 6B10dot2_PE_CD247_Antibody_ICFC_1_052115
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with Fixation Buffer and the Permeabilization buffer. Cells were then stained with CD247 (clone 6B10.2) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 6B10dot2_PE_CD247_Antibody_ICFC_2_052115
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644105 25 µg 153 CHF
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644106 100 µg 360 CHF
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Description

The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR heterodimer. The disulfide-linked TCR zeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TCR-zeta peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.2 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Zhang Z, et al. 2007. Blood 109:4328. (Block)
  2. Gorman CL, et al. 2008. J. Immunol. 180:1060.
  3. Jenson WA, et al. 1997. Eur J. Immunol. 27:707.
  4. Mao H, et al. 2010. J. Virol. 84:4148. PubMed
  5. Hwang I, et al. 2012. Int Immunol. 24:793. PubMed.
Product Citations
  1. Woan KV, et al. 2021. Cell Stem Cell. 28:2062. PubMed
  2. Lah S, et al. 2023. J Immunother Cancer. 11:. PubMed
  3. Hintz HM, et al. 2021. Cancer Immunol Res. 9:1270. PubMed
  4. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
RRID
AB_2565721 (BioLegend Cat. No. 644105)
AB_2565721 (BioLegend Cat. No. 644106)
Disclaimer

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Antigen Details

Structure
Structure: 163 amino acid long protein (predicted MW is 18kDa), with a short extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
Distribution

T cells and NKT cells. Reported also positive on CD16+ human NK cells.

Function
Mediating signal transduction after TCR receptor engagement.
Interaction
ZAP70, TCR alpha/beta
Cell Type
NKT cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules, TCRs
Antigen References

1.Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709

Gene ID
919 View all products for this Gene ID
UniProt
View information about CD247 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05.21.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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