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Purified anti-CD247 (TCRζ, CD3ζ) Antibody

Pricing & Availability
Clone
6B10.2 (See other available formats)
Regulatory Status
RUO
Other Names
CD247, CD3zeta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6B10point2_PURE_CD247_WB_050118
Total lysates (15 µg protein) from Jurkat, Daudi (low expression, negative control) and Raw264.7 were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 ug/ml) purified anti-CD247 antibody, clone 6B10.2. Proteins were visualized using chemiluminescence detection by incubation with and HRP goat anti-mouse IgG secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 6B10point2_PURE_CD247_WB_050118
    Total lysates (15 µg protein) from Jurkat, Daudi (low expression, negative control) and Raw264.7 were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 ug/ml) purified anti-CD247 antibody, clone 6B10.2. Proteins were visualized using chemiluminescence detection by incubation with and HRP goat anti-mouse IgG secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
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644101 25 µg 118 CHF
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644102 100 µg 293 CHF
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Description

The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR heterodimer. The disulfide-linked TCR zeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TCR-zeta peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-1 µg per ml antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhang Z, et al. 2007. Blood 109:4328. (Block)
  2. Gorman CL, et al. 2008. J. Immunol. 180:1060.
  3. Jenson WA, et al. 1997. Eur J. Immunol. 27:707.
  4. Mao H, et al. 2010. J. Virol. 84:4148. PubMed
  5. Hwang I, et al. 2012. Int Immunol. 24:793. PubMed.
Product Citations
  1. Wang X, et al. 2023. EMBO J. e110597:42. PubMed
  2. Ghosh S, et al. 2020. Cell Reports. 30(10):3434-3447. PubMed
  3. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  4. Hwang I, et al. 2012. Int Immunol. 24:793. PubMed
  5. Mao H, et al. 2010. J Virol. 84:4148. PubMed
RRID
AB_2072963 (BioLegend Cat. No. 644101)
AB_2072963 (BioLegend Cat. No. 644102)
Disclaimer

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Antigen Details

Structure
Structure: 163 amino acid long protein (predicted MW is 18kDa), with a short extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
Distribution

T cells and NKT cells. Reported also positive on CD16+ human NK cells.

Function
Mediating signal transduction after TCR receptor engagement.
Interaction
ZAP70, TCR alpha/beta
Cell Type
NKT cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules, TCRs
Antigen References

1.Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709

Gene ID
919 View all products for this Gene ID
UniProt
View information about CD247 on UniProt.org
Go To Top Version: 2    Revision Date: 05.01.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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