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- Clone
- W19011B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- G0/G1 switch regulatory protein 3 (G0S3), FosB Proto-Oncogene, AP-1 Transcription Factor Subunit, Oncogene Fos-B, FBJ Murine Osteosarcoma Viral Oncogene Homolog B
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
HeLa cells untreated (negative control, open histogram) or treated with 100 ng/ml PMA for 4 hours (positive control, filled histogram) were fixed and permeabilized using the True Phos™ Fix/Perm Buffer Set (Cat. No. 425401), and intracellularly stained with PE anti-FosB (clone W19011B) or PE rat IgG2a, κ isotype control (open histogram, dashed line) (representative histogram for untreated and treated cells) (Cat. No. 400507).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 600857 | 25 tests | 214 CHF | ||||
| 600858 | 100 tests | 420 CHF | ||||
FosB is a member of the FOS family of proteins that dimerizes with proteins of the Jun family to form the AP-1 transcription factor complex. As part of AP-1, FosB plays a critical role in regulating cell differentiation, proliferation, and apoptosis. FosB is expressed in most tissues and has been particularly well-studied in the CNS. ΔFosB, a truncated splice variant of the FOSB gene, plays a critical role in the brain’s reward circuit plasticity, with significant impact on both natural and pathological behavior. FosB also functions as a tumor suppressor, and dysregulation of FosB activity or levels has been found in numerous cancers, including gastric, prostate and colorectal cancer.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full-length recombinant FosB protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
For ICC, we recommend fixation in 4% PFA and permeabilization for 6 minutes in 0.5% Triton-X.
Methanol only and PFA + Methanol fix/perm methods were tested and produced inferior signal.
ICFC: Cells were fixed and permeabilized using True Phos Buffer system. - RRID
-
AB_2941601 (BioLegend Cat. No. 600857)
AB_2941601 (BioLegend Cat. No. 600858)
Antigen Details
- Structure
- FosB is a 338 amino acid protein with a predicted molecular weight of 36kD.
- Distribution
-
FosB is widely expressed in developing and adult tissues, including the brain, thyroid, bladder and adipose tissue.
- Function
- FosB heterodimerizes with proteins of the Jun family to form the transcription factor AP-1 to regulate cell proliferation, survival, and differentiation.
- Interaction
- Jun family proteins e.g. c-Jun, genes with the TPA response element (TGA G/C TCA) SWI/SNF chromatin remodeling complex, DPP4
- Cell Type
- Mature Neurons
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Neuroscience, Neuroscience Cell Markers, Transcription Factors
- Molecular Family
- Tumor Suppressors
- Antigen References
-
- Nestler E. 2015. Eur J Pharmacol. 753:66-72.
- Milde-Langosch K. 2005. Eur J Cancer. 41:2449-61.
- Gene ID
- 2354 View all products for this Gene ID
- UniProt
- View information about FosB on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All FosB Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-FosB | W19011B | IHC-P,ICC,ICFC |
| Alexa Fluor® 647 anti-FosB | W19011B | ICFC,ICC |
| PE anti-FosB | W19011B | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-FosB
IHC staining of purified anti-FosB (clone W19011B) on formal... 
ICC staining of purified anti-FosB (clone W19011B) (panel A)... 
Hela cells treated with 100 ng PMA for 4 hours (positive tar... 
Untreated HeLa cells (negative target) were fixed with Fixat... -
Alexa Fluor® 647 anti-FosB
HeLa cells untreated (panels A and B) or treated with 100 ng... 
HeLa cells untreated (negative control, open histogram) or t... -
PE anti-FosB
HeLa cells untreated (negative control, open histogram) or t...
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