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PE anti-human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
HI98 (See other available formats)
Regulatory Status
RUO
Workshop
IV M141
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
HI98_PE_061807
Human peripheral blood granulocytes stained with HI98 PE
  • HI98_PE_061807
    Human peripheral blood granulocytes stained with HI98 PE
  • HI98_PE_CD15_Antibody_042922
    Pre-lysed human blood leukocytes were stained with CD15 (Clone HI98) PE (filled histogram) or mouse IgM, κ PE Isotype Control (Open Histogram)

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
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Cat # Size Price Quantity Check Availability Save
301905 25 tests 113 CHF
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301906 100 tests 262 CHF
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections5 and formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. van Baarsen LG, et al. 2014. Arthritis Res. Ther. 16:426. (IHC)
Product Citations
  1. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  2. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  3. Zhao M, et al. 2021. Frontiers in Cell and Developmental Biology. 9:652972. PubMed
  4. Singh VK, et al. 2022. Front Immunol. 13:865503. PubMed
  5. Zhao M, et al. 2022. J Oncol. 2022:3345536. PubMed
  6. Jin J, et al. 2023. JCI Insight. 8:. PubMed
  7. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  8. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  9. Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed
  10. Carlucci PM, et al. 2018. JCI Insight. 3. PubMed
  11. Ma X, et al. 2022. Front Oncol. 12:855570. PubMed
  12. Xu Y, et al. 2021. Stem Cell Res Ther. 12:112. PubMed
  13. Nirschl TR, et al. 2020. Am J Clin Exp Urol. 8:48. PubMed
  14. Terenzi DC, et al. 2021. STAR Protoc. 2:100311. PubMed
  15. Li F, et al. 2022. Front Pharmacol. 13:1001552. PubMed
  16. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  17. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  18. Yao J, et al. 2022. Front Pharmacol. 13:876076. PubMed
  19. Jin J, et al. 2022. Cell Biosci. 12:114. PubMed
  20. Georgakis S, et al. 2021. JCI Insight. 6:. PubMed
  21. LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed
  22. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
  23. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  24. Gourvest M, et al. 2021. Leukemia. . PubMed
  25. Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
  26. Crippa S, et al. 2019. J Clin Invest. 129:1566. PubMed
  27. Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
  28. Qu M, et al. 2021. Frontiers in Oncology. 11:659720. PubMed
  29. Assadi G, et al. 2016. PLoS One. 11:e0168276. PubMed
RRID
AB_314198 (BioLegend Cat. No. 301905)
AB_314198 (BioLegend Cat. No. 301906)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Monocytes, Neural Stem Cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12.19.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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