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Purified anti-human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
HI98 (See other available formats)
Regulatory Status
RUO
Workshop
IV M141
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
1-HI98_Purified_061807
Human peripheral blood granulocytes stained with purified HI98, followed by anti-mouse IgGs FITC
  • 1-HI98_Purified_061807
    Human peripheral blood granulocytes stained with purified HI98, followed by anti-mouse IgGs FITC
  • 2-HI98_Pure_CD15_Antibody_2_103118
    Formalin-fixed paraffin-embedded human tonsil treated with a citrate buffer pH6 and heat for antigen retrieval was stained with purified CD15 clone HI98, conjugated and detected with a Cy5 conjugated CODEX™ oligonucleotide duplex (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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301902 100 µg 102 CHF
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections5 and formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. van Baarsen LG, et al. 2014. Arthritis Res. Ther. 16:426. (IHC)
Product Citations
  1. Tornack J, et al. 2017. PLoS One. 12:e0169119. PubMed
  2. de Vargas Roditi L, et al. 2022. Cell Rep Med. 3:100604. PubMed
  3. Dao T, et al. 2022. JCI Insight. 7: . PubMed
  4. Kirolos SA, et al. 2022. J Leukoc Biol. 112:1399. PubMed
  5. Fischer JR, et al. 2023. Cell Rep Med. 4:100977. PubMed
  6. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  7. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  8. Antonelou M, et al. 2020. J Am Soc Nephrol. 31:350. PubMed
  9. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  10. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  11. Xie S, et al. 2020. Ann Transl Med. 1.384027778. PubMed
  12. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  13. Schwabenland M, et al. 2021. Immunity. . PubMed
  14. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  15. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  16. Keskin DB, et al. 2019. Nature. 565:234. PubMed
  17. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  18. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
RRID
AB_314194 (BioLegend Cat. No. 301902)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Monocytes, Neural Stem Cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org
Go To Top Version: 3    Revision Date: 10.31.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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