PE anti-human CD163 Antibody anti-CD163

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Clone: RM3/1 (See other available formats)
Regulatory Status: RUO
Other Names: GHI/61, M130, RM3/1, p155, Hemoglobin/haptoglobin complex receptor, Macrophage-associated antigen, ED2(rat), Macrophage marker
Isotype: Mouse IgG1, κ
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Product Citations: publications

RM3slash1_PE_122007 — IL-10 stimulated human peripheral blood monocytes stained with RM3/1 PE

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326505

25 tests

122 CHF

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326506

100 tests

268 CHF

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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kuffer cells, monocytes, subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammation diseases.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human monocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone RM3/1 binds to domain 9 of CD163. Additional reported applications (for the relevant formats) include: immunofluorescence⁷.
RRID: AB_893265 (BioLegend Cat. No. 326505)
AB_893265 (BioLegend Cat. No. 326506)

Antigen Details

Structure: 134 kD (non-reduced)/155 kD (reduced) glycoprotein, Scavenger receptor superfamily
Distribution: Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells
Function: Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Ligand/Receptor: Haptoglobin-hemoglobin complex, TWEAK
Cell Type: Monocytes, Macrophages, Dendritic cells, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Roth J, et al. 1994. Transolantation. 57:127.
2. Van den Heuvel MM, et al.1999. J. Leukoc. Biol. 66:858.
3. Sulahian TH, et al. 2000. Cytokines 12:1312.
4. Fabriek BO, et al. 2007. J. Neuroimmunol. 187:179.
Gene ID: 9332 View all products for this Gene ID
UniProt: View information about CD163 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD163 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD163	RM3/1	FC,ICC
PE anti-human CD163	RM3/1	FC
Alexa Fluor® 647 anti-human CD163	RM3/1	FC
APC anti-human CD163	RM3/1	FC
PerCP/Cyanine5.5 anti-human CD163	RM3/1	FC
PE/Cyanine7 anti-human CD163	RM3/1	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 05.01.2014

For Research Use Only. Not for diagnostic or therapeutic use.

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