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PE anti-human Podoplanin Antibody

Pricing & Availability
Clone
NC-08 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, T1a2, gp36, gp38, gp40, Aggrus, PDPN, D2-40
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
NC-08_PE_042909.jpg
Human glioblastoma cell line LN319 stained with NC-08 PE
  • NC-08_PE_042909.jpg
    Human glioblastoma cell line LN319 stained with NC-08 PE
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Cat # Size Price Quantity Check Availability Save
337003 25 tests 153 CHF
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337004 100 tests 360 CHF
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Description

Podoplanin is a 40-43 kD type-I transmembrane sialomucin-type glycoprotein, also known as T1a, gp36, gp38, gp40, and Aggrus. Originally detected on the surface of podocytes, futher characterization showed podoplanin has a broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and a variety of tumor cells. It has been reported that podoplanin is the ligand of CLEC2 and is involved in lymphatic vessel formation, platelet aggregation, and tumor metastasis. Podoplanin may serve as a useful marker for tumor diagnosis and prognosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence1.

Application References

(PubMed link indicates BioLegend citation)
  1. Fujino N, et al. 2012. Am. J. Respir. Cell. Mol. Biol. 46:422. (FC, IF)
Product Citations
  1. Suresh V, et al. 2022. FASEB J. 36:e22449. PubMed
  2. Offeddu GS, et al. 2022. ALTEX. :. PubMed
  3. Guahmich NL, et al. 2023. Commun Biol. 6:7. PubMed
  4. Sasano T, et al. 2022. J Thromb Haemost. 20:104. PubMed
  5. Henry GH et al. 2018. Cell reports. 25(12):3530-3542 . PubMed
  6. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  7. Takeda A, et al. 2020. Immunity. 51(3):561-572. PubMed
  8. Eichin D, et al. 2020. Eur J Immunol. . PubMed
  9. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
  10. Takeda A, et al. 2020. STAR Protoc. 1:100021. PubMed
  11. Christianson D, et al. 2015. Proc Natl Acad Sci U S A. 112:2521. PubMed
  12. Rodrigues Toste de Carvalho AL, et al. 2021. Nat Protoc. 16:1802. PubMed
RRID
AB_1595457 (BioLegend Cat. No. 337003)
AB_1595457 (BioLegend Cat. No. 337004)

Antigen Details

Structure
40-43 kD type-I transmembrane sialomucin-type glycoprotein
Distribution

Broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and many tumor cells

Function
Lymphatic vessel formation, tumor metastasis, platelet aggregation
Ligand/Receptor
CLEC2
Cell Type
Dendritic cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Raica M, et al. 2008. Anticancer Res. 28:2997.
2. Xie Q, et al. 2008. Int. J. Clin. Exp. Pathol. 1:276.
3. Ogasawara S, et al. 2008. Hybridoma. 27:259.
4. Kato Y, et al. 2003. J. Bio. Chem. 278:51599.

Gene ID
10630 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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