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PE anti-mouse CD103 Antibody

Pricing & Availability
Clone
2E7 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE
Isotype
Armenian Hamster IgG
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Product Citations
publications
2E7_PE_081908.jpg
C57BL/6 splenocytes stained with 2E7 PE
  • 2E7_PE_081908.jpg
    C57BL/6 splenocytes stained with 2E7 PE
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121405 25 µg 73 CHF
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121406 100 µg 219 CHF
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Description

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse intestinal intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.

Application References

(PubMed link indicates BioLegend citation)
  1. LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP)
  2. Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF)
  3. Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed
  4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  5. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  6. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
  7. Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)
Product Citations
  1. Parsons M, et al. 2014. J Immunol. 192:1361. PubMed
  2. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  3. Bruand M, et al. 2021. Cell Reports. 36(3):109412. PubMed
  4. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  5. Oliver AJ, et al. 2020. Oncoimmunology. 9:1802979. PubMed
  6. Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed
  7. Klaus T, et al. 2022. JCI Insight. 7: . PubMed
  8. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  9. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  10. Tregoning JS, et al. 2023. Mol Ther Nucleic Acids. 31:29. PubMed
  11. Cheng K, et al. 2023. Small. 19:e2300125. PubMed
  12. Shi X, et al. 2023. iScience. 26:106143. PubMed
  13. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  14. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  15. Latour YL, et al. 2023. Gut Microbes. 15:2192623. PubMed
  16. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
  17. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  18. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  19. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  20. Vogel AJ et al. 2015. Frontiers in immunology. 0.477083333 . PubMed
  21. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  22. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  23. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  24. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  25. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  26. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  27. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  28. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  29. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  30. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  31. Hao F, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  32. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  33. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  34. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  35. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  36. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  37. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  38. Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed
  39. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  40. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  41. Crooks SD, et al. 2022. Immunohorizons. 6:705. PubMed
  42. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
  43. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  44. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  45. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  46. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  47. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  48. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  49. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  50. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  51. Guo S, et al. 2021. Sci Rep. 11:23745. PubMed
  52. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  53. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  54. Zhang XW, et al. 2021. Oncoimmunology. 10:1920739. PubMed
  55. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  56. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  57. Carlson PM, et al. 2021. J Immunol. 207:720. PubMed
  58. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  59. Yamazaki S, et al. 2015. J Immunol. 195: 3427 - 3435. PubMed
  60. Lee HY, et al. 2021. Curr Protoc. 1:e272. PubMed
  61. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  62. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  63. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  64. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  65. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  66. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  67. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
  68. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  69. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
RRID
AB_535948 (BioLegend Cat. No. 121405)
AB_535948 (BioLegend Cat. No. 121406)

Antigen Details

Structure
Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution

Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.

Function
Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor
E-Cadherin
Cell Type
Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.

Gene ID
16407 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 05.04.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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