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PE anti-mouse CD157 (BST-1) Antibody

Pricing & Availability
Clone
BP-3 (See other available formats)
Regulatory Status
RUO
Other Names
cADPr hydrolase 2, Ly-65, BP-3, ADP-ribosyl cyclase 2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
BP-3_PE_102110
BALB/c bone marrow cells were stained with CD157 (BP-3) PE and CD45R/B220 (RA3-6B2) APC.
  • BP-3_PE_102110
    BALB/c bone marrow cells were stained with CD157 (BP-3) PE and CD45R/B220 (RA3-6B2) APC.
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140203 25 µg 104 CHF
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140204 100 µg 292 CHF
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Description

CD157 is an ectoenzyme member of the ADP-ribosyl cyclase family, also known as ADP-ribosyl cyclase 2, BST-1 (bone marrow stromal cell antigen 1), BP-3 antigen. It has a molecular weight between 40 - 46 kD and is a GPI-linked cell membrane glycoprotein which is expressed as a homodimer. CD157 is expressed by macrophages, neutrophils, bone marrow stromal cells, endothelial cells, follicular dendritic cells, and T and B cell progenitors prior to the rearrangement of the antigen receptors. Besides the ectoenzyme activity, it also plays a role in neutrophil migration and adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Murine pre-B cell line 18.81
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. McNagny KM, et al. 1988. J. Immunol. 141:2551. (FC)
Product Citations
  1. Wu TJ, et al. 2022. Cell Mol Life Sci. 79:348. PubMed
  2. Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed
  3. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  4. Wakabayashi T, et al. 2022. Invest Ophthalmol Vis Sci. 63:5. PubMed
  5. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  6. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
RRID
AB_10643273 (BioLegend Cat. No. 140203)
AB_10643273 (BioLegend Cat. No. 140204)

Antigen Details

Structure
Member of the ADP-ribosyl cyclase family, ectoenzyme, 40-46 kD, glycosyl-phosphatidylinositol (GPI)- anchored membrane protein, present on the cell surface as a homodimer
Distribution

Expressed by endothelial cells, follicular dendritic cells, cells from the myeloid lineage, and T and B cell precursors prior to the rearrangement of the antigen receptor. Expressed in many cell types from different tissues like the bone marrow, spleen, thymus, lung, kidney and heart.

Function
Synthesizes cyclic ADP-ribose (cADPR), a second messenger that elicits calcium release from intracellular stores.
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Yamamoto-Katayama S, et al. 2002. J. Mol. Biol. 310:711.
2. Funaro A, et al. 2009. Front. Biosci. 14:929.
3. Malavasi F, et al. 2006. Mol. Med. 12:334.
4. Vicari AP, et al. 1996. Int. Immunol. 8:183.

Gene ID
12182 View all products for this Gene ID
UniProt
View information about CD157 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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