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PE anti-mouse CD23 Antibody

Pricing & Availability
Clone
B3B4 (See other available formats)
Regulatory Status
RUO
Other Names
FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
B3B4_PE_071608.jpg
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) PE (filled histogram), or rat IgG2a, κ PE (open histogram).
  • B3B4_PE_071608.jpg
    CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) PE (filled histogram), or rat IgG2a, κ PE (open histogram).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
101607 50 µg 110 CHF
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101608 200 µg 293 CHF
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Description

CD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, immunohistochemical staining of acetone-fixed frozen sections5, and spatial biology (IBEX)7,8.

Application References

(PubMed link indicates BioLegend citation)
  1. Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
  2. Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
  3. Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
  4. Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
  5. Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
  6. Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  2. Chiu H, et al. 2021. iScience. 24:102748. PubMed
  3. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  4. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  5. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  6. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  7. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  8. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  9. Lee-Chang C, et al. 2016. J Immunol. 196: 3385-3397. PubMed
  10. McGuire A, et al. 2016. Nat Commun. 7:10618. PubMed
  11. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  12. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  13. Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed
  14. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  15. Zhao X, et al. 2017. Nat Med. 10.1038/nm.4260. PubMed
  16. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  17. Aschman T, et al. 2021. Int J Mol Sci. 22:. PubMed
  18. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  19. Wang D, et al. 2005. Int Immunol. 1.527083333. PubMed
  20. Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed
  21. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  22. Escolano A, et al. 2016. Cell. 166(6):1445-1458.e12. PubMed
  23. Smith LK, et al. 2021. Elife. 10:. PubMed
  24. Haines RR, et al. 2019. J Immunol. 203:1867. PubMed
  25. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  26. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
RRID
AB_312832 (BioLegend Cat. No. 101607)
AB_312832 (BioLegend Cat. No. 101608)

Antigen Details

Structure
C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution

B cells, follicular dendritic cells

Function
Regulates B cell activation
Ligand/Receptor
IgE, CD19/CD21/CD81
Cell Type
B cells, Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.

Gene ID
14128 View all products for this Gene ID
UniProt
View information about CD23 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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