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PE anti-mouse CD283 (TLR3) Antibody

Pricing & Availability
Clone
11F8 (See other available formats)
Regulatory Status
RUO
Other Names
Toll like receptor 3
Isotype
Rat IgG2a
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Product Citations
publications
11F8_PE_1_082911
C57BL/6 mouse splenocytes were surface stained with CD4/CD19/Ly-6G/NK1.1 PerCP, CD11c APC and CD8 FITC, followed by intracellular staining with TLR3 (clone 11F8) PE (top) or rat IgG2a, κ PE isotype control (bottom). The data was analyzed by gating on CD4/CD19/Ly-6G/NK1.1 negative and CD8 positive cells.
  • 11F8_PE_1_082911
    C57BL/6 mouse splenocytes were surface stained with CD4/CD19/Ly-6G/NK1.1 PerCP, CD11c APC and CD8 FITC, followed by intracellular staining with TLR3 (clone 11F8) PE (top) or rat IgG2a, κ PE isotype control (bottom). The data was analyzed by gating on CD4/CD19/Ly-6G/NK1.1 negative and CD8 positive cells.
  • 11F8_PE_2_082911
See PE spectral data
Cat # Size Price Quantity Check Availability Save
141903 25 µg 177 CHF
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141904 100 µg 360 CHF
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Description

Toll-like receptor 3 (TLR3), also known as CD283, is type I transmembrane protein that belongs to the TLR family. It forms a large horseshoe shape that contacts a neighboring horseshoe, forming a dimer of two horseshoes. It is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. TLR3 is expressed selectively on the cytoplasmic membrane and intracellularly in dendritic cells. It is also highly expressed in the placenta, pancreas, heart, liver, lung, and muscle. TLR3 is a pattern recognition receptor that participates in innate immune response to microbial pathogens by recognizing polyinosine-polycytidylic acid (Poly(I:C)) and dsRNA. Ligand binding by TLR3 induces receptor dimerization which results in inducing NF-κB activation (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 has been shown to interact with a number of proteins including MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse TLR3 extracellular domain.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue1.

Application References

(PubMed link indicates BioLegend citation)
  1. McCartney S, et al. 2009. J. Exp. Med. 13:2967. (IHC)
Product Citations
  1. Chimote AA, et al. 2016. J Autoimmun. 86:69. PubMed
  2. Stone AEL, et al. 2019. Nat Commun. 10:3649. PubMed
  3. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
RRID
AB_10895749 (BioLegend Cat. No. 141903)
AB_10895749 (BioLegend Cat. No. 141904)

Antigen Details

Structure
Toll-like receptor family member; type I membrane protein containing multiple leucine repeats and a interleukin-1 receptor like region in the extracellular domain. Predicted molecular weight approximately 116 kD.
Distribution
Selective expression in dendritic cells. Highly expressed in placenta and the pancreas; also expressed in heart, liver, lung and muscle.
Function
Pattern recognition receptor that participates in innate immune response to microbial pathogens. TLR3 ligands induce NF-κB (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 stimulation results in the production of cytokines.
Interaction
MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2.
Ligand/Receptor
Polyinosine-polycytidylic acid (Poly(I:C)), dsRNA.
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Alexopoulou L, et al. 2001. Nature 413:732.
2. Doyle SE, et al. 2002. Immunity 17:251.
3. Meylan E, et al. 2004. Nat Immunol. 5:503.
4. Muzio M, et al. 2000. J. Immunol. 164:5998.
5. Rock FL, et al. 1998. P. Natl. Acad. Sci. USA 95:588.

Gene ID
142980 View all products for this Gene ID
UniProt
View information about CD283 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 04.14.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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