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PE anti-mouse CD369 (Dectin-1/CLEC7A) Antibody

Pricing & Availability
Clone
RH1 (See other available formats)
Regulatory Status
RUO
Other Names
BGR, βGR, CD369
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
RH1_PE_081612
Thioglycolate-induced C57BL/6 mouse peritoneal cells were stained with anti-mouse CD369 (Dectin-1/CLEC7A, clone RH1) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram). Data shown was gated on the F4/80-positive macrophage population.
  • RH1_PE_081612
    Thioglycolate-induced C57BL/6 mouse peritoneal cells were stained with anti-mouse CD369 (Dectin-1/CLEC7A, clone RH1) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram). Data shown was gated on the F4/80-positive macrophage population.
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144303 25 µg 117 CHF
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144304 100 µg 335 CHF
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Description

CD369 (Dectin-1), also known as CLEC7A, is a 43 kD type II transmembrane protein.  The extracellular portion of Dectin-1 contains one C-type lectin domain, and its cytoplasmic tail has one immunoreceptor tyrosine-based activation motif (ITAM). Dectin-1 binds β-1,3-linked and β-1,6-glucans (abundant in the cell wall of fungi and yeast), mediates phagocytosis, and induces cytokine production and oxidative burst. It is also involved in TLR2-mediated NF-kB activation. Dectin-1 is expressed by macrophages, neutrophils, dendritic, and Langerhans cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Cansever D, et al. 2023. Nat Immunol. . PubMed
  2. Jaiswal AK, et al. 2020. Immunobiology. 225:151956. PubMed
  3. Lee S, et al. 2015. PLoS One. 10: 0132329. PubMed
  4. Chen S, et al. 2018. J Biol Rhythms. 33:99. PubMed
  5. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  6. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  7. Zhao Y, et al. 2016. Nat Commun. 7:12368. PubMed
RRID
AB_2561500 (BioLegend Cat. No. 144303)
AB_2561500 (BioLegend Cat. No. 144304)

Antigen Details

Structure
43 kD, type II transmembrane protein, 1 C-type lectin domain, 1 ITAM motif
Distribution
Macrophages, dendritic cells, Langerhans cells
Function
Phagocytosis, induces cytokine production and oxidative burst
Ligand/Receptor
β-1,3-linked and β-1,6-linked glucans
Cell Type
Macrophages, Dendritic cells, Langerhans cells, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Iliev ID, et al. 2012. Science 336:1314.
2. Saijo S and Iwakura Y. 2011. Int. Immunol. 23:467.
3. Reid DM, et al. 2009. Curr. Opin. Immunol. 21:30.
4. Saijo S, et al. 2007. Nat. Immunol. 8:39.

Gene ID
56644 View all products for this Gene ID
UniProt
View information about CD369 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 01.16.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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