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PE anti-mouse CD40 Antibody

Pricing & Availability
Clone
FGK45 (See other available formats)
Regulatory Status
RUO
Other Names
Bp50, TNFRSF5
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
FGK45_PE_CD40_Antibody_030220
C57BL/6 mouse splenocytes were stained with APC CD3 and PE CD40 (clone FGK45) (left) or PE rat IgG2a, κ isotype (right).
  • FGK45_PE_CD40_Antibody_030220
    C57BL/6 mouse splenocytes were stained with APC CD3 and PE CD40 (clone FGK45) (left) or PE rat IgG2a, κ isotype (right).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
157505 25 µg 83 CHF
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157506 100 µg 248 CHF
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Description

CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD40 – Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The FGK45 antibody can block the binding of HM40-3 clone. The FGK45 antibody can also stimulate mouse B cell proliferation.

Application References

(PubMed link indicates BioLegend citation)
  1. Andersson J, et al. 1996. Immunity 5:319-330.
  2. Melief CJ, et al. 1998. Nature 393(6684):480-3.
  3. Londei M, et al. 2000. Nat Med 6(6):673-9.
  4. Tominaga T, et al. 2015. Neuro Oncol 17(11):1453-62.
Product Citations
  1. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  2. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
RRID
AB_2832552 (BioLegend Cat. No. 157505)
AB_2832552 (BioLegend Cat. No. 157506)

Antigen Details

Structure
48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
Distribution

Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.

Function
Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
Interaction
TRAF molecules
Ligand/Receptor
CD154 (CD40L)
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
  3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
  4. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.
Gene ID
21939 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03.02.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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