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PE anti-mouse CD93 (AA4.1, early B lineage) Antibody

Pricing & Availability
Clone
AA4.1 (See other available formats)
Regulatory Status
RUO
Other Names
AA4.1, C1qRp, Early B lineage antigen
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_AA4dot1_PE_010510
C57BL/6 mouse bone marrow cells stained with CD45R/B220 (RA3-6B2) APC and AA4.1 PE
  • A_AA4dot1_PE_010510
    C57BL/6 mouse bone marrow cells stained with CD45R/B220 (RA3-6B2) APC and AA4.1 PE
  • B_AA4dot1_PE_010510
    C57BL/6 mouse bone marrow cells stained with CD45R/B220 (RA3-6B2) APC and rat IgG2b (RTK4530) PE isotype control
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136503 50 µg 110 CHF
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136504 200 µg 317 CHF
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Description

CD93 is a 130-140kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen. It is a receptor expressed on immature B lymphocytes, hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, embryonic yolk sac. CD93 expression level on splenic immature/transitional B cells is much lower than in bone marrow. It is reinduced during plasma cell differentiation and plays an important role in maintaining plasma cells in bone marrow niches. Immature dendritic cells express CD93 and down-regulate this molecule upon maturation, suggesting a role in uptake of particles by DC. It is also expressed on monocytes, macrophages, and endothelial cells. Macrophages from CD93 (-/-) mice had a significant phagocytic defect in the clearance of apoptotic cells in vivo, indicating CD93 may contribute to the in vivo clearance of dying cells. Binding of CD93 to C1q remains controversial.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Pre-B lymphoma 70Z/3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. McKearn JP, et al. 1984. J. Immunol. 132:332
  2. Hanihara-Tatsuzaawa F, et al. 2014. J Biol Chem. 289:30925. PubMed
Product Citations
  1. Meyer SN, et al. 2020. Immunity. 51(3):535-547. PubMed
  2. Moreau J, et al. 2015. Blood. 126: 1184-1192. PubMed
  3. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  4. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  5. Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed
  6. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  7. Yankova E, et al. 2021. Nature. 593:597. PubMed
  8. Fredriksson-Lidman K, et al. 2017. PLoS One. 10.1371/journal.pone.0185509. PubMed
  9. Hanihara-Tatsuzawa F, et al. 2014. J Biol Chem. 389:30925. PubMed
  10. Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed
  11. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  12. Silva N, et al. 2016. J Immunol. 196: 2591 - 2601. PubMed
RRID
AB_1967125 (BioLegend Cat. No. 136503)
AB_1967125 (BioLegend Cat. No. 136504)

Antigen Details

Structure
A 130-140 kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen.
Distribution

Expressed during immature B lymphocytes, hematopoietic progenitors and stem cells in bone marrow, immature dendritic cells, monocytes, macrophages, and endothelial cells.

Function
CD93 plays an important role in maintaining of plasma cells in bone marrow niches. It may play a role in uptake of particles by DC and the in vivo clearance of dying cells by macrophages.
Cell Type
B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Steinberger P, et al. 2002. J. Leukoc. Biol. 71:133
2. Chevrier S, et al. 2009. Proc. Nat. Acad. Sci. U. S. A. 106:3895
3. Norsworthy PJ, et al. 2004. J. Immunol. 172:3406
4. Li YS, et al. 1996. Immunity 5:527
5. Szilvassy SJ, et al. 1993. Blood 81:2310

Gene ID
17064 View all products for this Gene ID
UniProt
View information about CD93 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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