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PE anti-mouse Ig light chain λ Antibody

Pricing & Availability
Clone
RML-42 (See other available formats)
Regulatory Status
RUO
Other Names
Imunoglobulin light chain lambda
Isotype
Rat IgG2a, κ
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Product Citations
publications
RML-42_PE_021009.jpg
Balb/c mouse splenocytes stained with RML-42 PE
  • RML-42_PE_021009.jpg
    Balb/c mouse splenocytes stained with RML-42 PE
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Cat # Size Price Quantity Check Availability Save
407307 50 µg 110 CHF
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407308 200 µg 323 CHF
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Description

The RML-42 monoclonal antibody reacts with immunoglobulin light chain lambda in all tested mouse haplotype (Igh-a and b). It does not react with kappa chain. The RML-42 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Ota M, et al. 2010. J. Immunol. 185:4128. PubMed
  2. Xu Z, et al. 2012. J. Immunol. 189:4470. PubMed
Product Citations
  1. Pioli KT, et al. 2023. iScience. 26:106223. PubMed
  2. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
  3. Tian M, et al. 2020. Proc Natl Acad Sci U S A. 117:7929. PubMed
  4. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  5. McGuire A, et al. 2016. Nat Commun. 7:10618. PubMed
  6. Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed
  7. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  8. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  9. Escolano A, et al. 2016. Cell. 166(6):1445-1458.e12. PubMed
RRID
AB_1027659 (BioLegend Cat. No. 407307)
AB_1027659 (BioLegend Cat. No. 407308)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 02.15.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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