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PE/Cyanine7 anti-mouse CD83 Antibody

Pricing & Availability
Clone
Michel-19 (See other available formats)
Regulatory Status
RUO
Other Names
HB15
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
Michael-19_PECy7_CD83_Antibody_FC_100615
Mouse CD83 transfected cells were stained with CD83 (clone Michel-19) PE/Cyanine7 (filled histogram), or rat IgG1, κ PE/Cyanine7 isotype control (open histogram).
  • Michael-19_PECy7_CD83_Antibody_FC_100615
    Mouse CD83 transfected cells were stained with CD83 (clone Michel-19) PE/Cyanine7 (filled histogram), or rat IgG1, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
121517 25 µg 147 CHF
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121518 100 µg 358 CHF
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Description

CD83 is a 45 kD type I transmembrane protein. It belongs to immunoglobulin superfamily and is expressed on mature dendritic cells and activated lymphocytes. CD83 is involved in the regulation of T cell development and immune response. Soluble form CD83 has been reported to inhibit dendritic cell maturation and dendritic cell-mediated T cell proliferation. Murine CD83 ligand has been found on B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD83 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone - fixed frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Cramer SO, et al. 2000. Int. Immunol. 12:1347.
  2. Fujimoto Y, et al. 2002. Cell 108:755.
  3. Mott KR, et al. 2009. Virol J. 6:56. (FC) PubMed
  4. Roland Cl, et al. 2009. Mol Cancer Res. 8:1761. (IHC) PubMed
  5. Masuda Y, et al. 2010. Cancer Immunol Immunother. [Epub ahead of print] (FC) PubMed
  6. Tze LE, et al. 2011. J Exp Med. PubMed
  7. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  2. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  3. He Q, et al. 2019. Jpn J Infect Dis. 73:111. PubMed
RRID
AB_2566123 (BioLegend Cat. No. 121517)
AB_2566123 (BioLegend Cat. No. 121518)

Antigen Details

Structure
Type I transmembrane protein, Ig superfamily, 45kD
Distribution

Mature dendritic cells, activated lymphocytes

Function
Regulate T cell development and immune response, soluble form CD83 inhibit dendritic cell maturation
Ligand/Receptor
CD83 ligand
Cell Type
Dendritic cells, Lymphocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Lechmann M, et al. 2005. Biochem. Biophys. Res. Commun. 329:132.
2. Kotxor N, et al. 2004. Immunobiology 209:129.
3. Leon F, et al. 2004. J. Immunol. 173:2995.
4. Cramer SO, et al. 2000. Int. Immunol. 12:1347.
5. Fujimoto Y, et al. 2002. Cell 108:755.

Gene ID
12522 View all products for this Gene ID
UniProt
View information about CD83 on UniProt.org
Go To Top Version: 1    Revision Date: 10.06.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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