PE/Cyanine7 anti-mouse TCR DO11.10 Antibody

Pricing & Availability
Clone
KJ1-26 (See other available formats)
Regulatory Status
RUO
Other Names
DO11.10 Clonotypic T Cell Receptor
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
KJ1-26_PECy7_TCRDO11.10 _Antibody_FC_080113.jpg
DO11.10 T-hybridoma cells were stained with TCR DO11.10 (clone KJ1-26) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).
  • KJ1-26_PECy7_TCRDO11.10 _Antibody_FC_080113.jpg
    DO11.10 T-hybridoma cells were stained with TCR DO11.10 (clone KJ1-26) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
118515 25 µg 124 CHF
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118516 100 µg 335 CHF
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Description

DO11.10 transgenic TCR is specific for the chicken ovalbumin peptide (a.a. 323-339) presented on the I-Ad MHC class II complex. KJ1-26 antibody clone recognizes the DO11.10 TCR and enables identification of the DO11.10 TCR positive T-cells in the DO11.10 TCR transgenic mouse model. KJ1-26 antibody clone was reported to block the antigen response of DO11.10 T cell hybridomas in vitro.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DO11.10 T hybridoma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Haskins K, et al. 1983. J. Exp. Med. 157:1149.
  2. Martin RK, et al. 2014. PLoS One. 9:110609. PubMed
Product Citations
  1. Gutierrez S, et al. 2021. PLoS Pathog. 17:e1009943. PubMed
RRID
AB_2562392 (BioLegend Cat. No. 118515)
AB_2562392 (BioLegend Cat. No. 118516)

Antigen Details

Structure
80-90 kD TCR expressed on DO11.10 T hybridomas
Distribution

Clonotypic TCR

Ligand/Receptor
Ovalbumin peptide (aa 323-339) in the context of mouse MHC class II I-Ad
Biology Area
Immunology, Adaptive Immunity
Molecular Family
TCRs
Antigen References

1. Haskins K, et al. 1983. J. Exp. Med. 157:1149.
2. Marrack P, et al. 1983. J. Exp. Med. 158:1635.

Gene ID
21473 View all products for this Gene ID
UniProt
View information about TCR DO11.10 on UniProt.org
Go To Top Version: 1    Revision Date: 08.09.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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