PerCP/Cyanine5.5 anti-mouse TCR DO11.10 Antibody anti-TCR DO11.10

Pricing & Availability

Clone: KJ1-26 (See other available formats)
Regulatory Status: RUO
Other Names: DO11.10 Clonotypic T Cell Receptor
Isotype: Mouse IgG2a, κ
Ave. Rating: Submit a Review
Product Citations: publications

KJ1-26_PerCPCy55_020110 — DO11.10 cells stained with KJ1-26 PerCP/Cyanine5.5

Compare all formats See PerCP/Cyanine5.5 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

118512

25 µg

147 CHF

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

DO11.10 transgenic TCR is specific for the chicken ovalbumin peptide (a.a. 323-339) presented on the I-A^d MHC class II complex. KJ1-26 antibody clone recognizes the DO11.10 TCR and enables identification of the DO11.10 TCR positive T-cells in the DO11.10 TCR transgenic mouse model. KJ1-26 antibody clone was reported to block the antigen response of DO11.10 T cell hybridomas in vitro.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: DO11.10 T hybridoma cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2028522 (BioLegend Cat. No. 118512)

Antigen Details

Structure: 80-90 kD TCR expressed on DO11.10 T hybridomas
Distribution: Clonotypic TCR
Ligand/Receptor: Ovalbumin peptide (aa 323-339) in the context of mouse MHC class II I-A^d
Biology Area: Immunology, Adaptive Immunity
Molecular Family: TCRs
Antigen References: 1. Haskins K, et al. 1983. J. Exp. Med. 157:1149.
2. Marrack P, et al. 1983. J. Exp. Med. 158:1635.
Gene ID: 21473 View all products for this Gene ID
UniProt: View information about TCR DO11.10 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All TCR DO11.10 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse TCR DO11.10	KJ1-26	FC
PE anti-mouse TCR DO11.10	KJ1-26	FC
PerCP/Cyanine5.5 anti-mouse TCR DO11.10	KJ1-26	FC
PE/Cyanine7 anti-mouse TCR DO11.10	KJ1-26	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.