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PerCP/Cyanine5.5 anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
PK136_PerCPCyanine55_NK11_Antibody_101818
C57BL/6 mouse splenocytes were stained with CD49b (pan-NK cells) APC and NK1.1 (clone PK136) PerCP/Cyanine5.5 (left), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • PK136_PerCPCyanine55_NK11_Antibody_101818
    C57BL/6 mouse splenocytes were stained with CD49b (pan-NK cells) APC and NK1.1 (clone PK136) PerCP/Cyanine5.5 (left), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
108727 25 µg 93 CHF
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108728 100 µg 319 CHF
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Minute L, et al. 2020. J Immunother Cancer. 8:. PubMed
  2. Ning J, et al. 2022. Cancer Immunol Immunother. 71:1863. PubMed
  3. VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed
  4. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  5. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  6. Leonardi I, et al. 2022. Cell. 185:831. PubMed
  7. Xiao R, et al. 2022. J Immunol. 208:2109. PubMed
  8. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  9. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  10. Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed
  11. Harpur CM, et al. 2023. Clin Transl Immunology. 12:e1443. PubMed
  12. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  13. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  14. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  15. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  16. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  17. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  18. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  19. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  20. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  21. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  22. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  23. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  24. Park D, et al. 2020. Cancer Res. 80:4172. PubMed
  25. Kooreman NG et al. 2018. Cell stem cell. 22(4):501-513 . PubMed
  26. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  27. Abdul Pari AA, et al. 2020. Cancer Res. 80:2586. PubMed
  28. Peauroi EM, et al. 2022. iScience. 25:105510. PubMed
  29. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  30. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  31. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  32. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  33. Tomala J, et al. 2020. Methods Mol Biol. 2111:101. PubMed
  34. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  35. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  36. Liyanage S, et al. 2016. Exp Eye Res. 151:160-70. PubMed
  37. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  38. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  39. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  40. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  41. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  42. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  43. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  44. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  45. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  46. Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
  47. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  48. Gardner P, et al. 2017. Sci Rep. 7:40830. PubMed
  49. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  50. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  51. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  52. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  53. Gawish R, et al. 2022. Elife. 11:. PubMed
  54. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  55. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  56. Tian L, et al. 2012. J Autoimmun. 38:39. PubMed
  57. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
RRID
AB_2132705 (BioLegend Cat. No. 108727)
AB_2132705 (BioLegend Cat. No. 108728)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All NK-1.1 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse NK-1.1 PK136 FC
Biotin anti-mouse NK-1.1 PK136 FC
FITC anti-mouse NK-1.1 PK136 FC
PE anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
PE/Cyanine7 anti-mouse NK-1.1 PK136 FC
PE/Cyanine5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 488 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 647 anti-mouse NK-1.1 PK136 FC
Pacific Blue™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 711™ anti-mouse NK-1.1 PK136 FC
APC/Cyanine7 anti-mouse NK-1.1 PK136 FC
PerCP anti-mouse NK-1.1 PK136 FC
PerCP/Cyanine5.5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 700 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 421™ anti-mouse NK-1.1 PK136 FC,SB
Brilliant Violet 570™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 650™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 510™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 605™ anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 (Maxpar® Ready) PK136 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 785™ anti-mouse NK-1.1 PK136 FC
APC/Fire™ 750 anti-mouse NK-1.1 PK136 FC
TotalSeq™-A0118 anti-mouse NK-1.1 PK136 PG
Ultra-LEAF™ Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
TotalSeq™-B0118 anti-mouse NK-1.1 PK136 PG
TotalSeq™-C0118 anti-mouse NK-1.1 PK136 PG
PE/Fire™ 810 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 640 anti-mouse NK-1.1 PK136 FC
Spark UV™ 387 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 700 anti-mouse NK-1.1 PK136 FC
Go To Top Version: 3    Revision Date: 10.18.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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