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PerCP/Cyanine5.5 anti-RANTES (CCL5) Antibody

Pricing & Availability
Clone
VL1 (See other available formats)
Regulatory Status
RUO
Other Names
CCL5, C-C motif chemokine 5, Small-inducible cytokine A5, T-cell-specific protein RANTES, SIS-delta, T cell-specific protein P228, TCP228, Eosinophil-chemotactic cytokine, EoCP
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
VL1_PerCPCyanine55_RANTES_Antibody_013019
Overnight cultured human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 FITC, then fixed, permeabilized, and intracellularly stained with RANTES (CCL5) (clone VL1) PerCP/Cyanine5.5 (left) or mouse IgG2b, κ PerCP/Cya
  • VL1_PerCPCyanine55_RANTES_Antibody_013019
    Overnight cultured human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 FITC, then fixed, permeabilized, and intracellularly stained with RANTES (CCL5) (clone VL1) PerCP/Cyanine5.5 (left) or mouse IgG2b, κ PerCP/Cya
Compare all formats See PerCP/Cyanine5.5 spectral data
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515507 25 tests 176 CHF
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515508 100 tests 382 CHF
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Description

RANTES (CCL5) is a member of the CC chemokine family. Its mature form consists of 68 amino acid residues. RANTES is a strong chemotractant for monocytes, NK cells, T cells, and eosinphils. RANTES may have important role in mediating immune and inflammatory responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Krensky AM, et al. 1997. Methods Enzymol. 287:162.
Product Citations
  1. Zhang M, et al. 2023. JCI Insight. 8: . PubMed
  2. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  3. Tao P, et al. 2021. Sci Adv. 7:eabi6794. PubMed
RRID
AB_2562665 (BioLegend Cat. No. 515507)
AB_2562665 (BioLegend Cat. No. 515508)

Antigen Details

Structure
Human RANTES cDNA is a member of the C-C chemokine family with a MW of around 8 kD. Human and mouse RANTES have 85% homology.
Distribution

Reported to be produced by T cells, platelets, synovial fibroblasts, and some tumor cells.

Function
Able to chemoattract monocytes, NK cells, eosinophils, and T cells.
Interaction
Monocytes, NK cells, T cells, and eosinophils.
Cell Type
Fibroblasts, Platelets, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Von Luettichau. et al. 1996. Cytokine 8:89.
2. Schall TJ. 1991. Cytokine 3:165.

Gene ID
20304 View all products for this Gene ID
UniProt
View information about RANTES on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 01.30.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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