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Purified anti-Brevican Antibody (Previously Covance catalog# MMS-5127)

Pricing & Availability
Clone
N294A/6 (See other available formats)
Regulatory Status
RUO
Other Names
Brevican core protein, BEHAB, brain-enriched hyaluronan-binding protein, brevican (brain specific proteoglycan in the aggrecan family)
Previously
Covance Catalog# MMS-5127
Isotype
Mouse IgG2b
Ave. Rating
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Product Citations
publications
N294Aslash6_Purified_Brevican_Antibody_1_WB_030415
Western blot of purified anti-Brevican antibody (clone N294A/6). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N294Aslash6_Purified_Brevican_Antibody_1_WB_030415
    Western blot of purified anti-Brevican antibody (clone N294A/6). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N294Aslash6_Purified_Brevican_Antibody_2_IHC_030415
    IHC staining of purified anti-Brevican antibody (clone N294A/6) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N294Aslash6_Purified_Brevican_Antibody_3_IHC_030415
    IHC staining of purified anti-Brevican antibody (clone N294A/6) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N294Aslash6_Purified_Brevican_Antibody_4_IHC_062118
    IHC staining of purified anti-Brevican antibody (clone N294A/6) on frozen rat brain tissue. The tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
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820101 100 µL 165 CHF
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Description

Brain-specific proteoglycan in the aggrecan family (brevican), also known as brain-enriched hyaluronan-binding protein (BEHAB), is a brain chondroitin sulfate proteoglycan specifically expressed in the central nervous system. It plays a role in development and formation of the brain extracellular matrix and may be involved in malignancy of brain tumor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 219-655 of rat Brevican.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For western blotting, the suggested use of this reagent is 5.0 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded, a concentration range of 1.0 - 10 µg/mL is suggested. For immunohistochemistry on frozen tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Rowlands D, et al. 2018. J Neurosci. 38:10102. PubMed
  2. Yamasaki S, et al. 2022. iScience. 25:103657. PubMed
  3. Nguyen PT, et al. 2020. Cell. 182(2):388-403.e15. PubMed
RRID
AB_2564837 (BioLegend Cat. No. 820101)

Antigen Details

Structure
Brevican is a 911 amino acid protein with an expected molecular weight of 140 kD; proteolyticaly cleaved fragment of Brevican may be observed at ~50 kD in some tissue lysates
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Gene ID
25393 View all products for this Gene ID
UniProt
View information about Brevican on UniProt.org
Go To Top Version: 5    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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