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Purified anti-CD29 (Integrin β1) Antibody

Pricing & Availability
Clone
P5D2 (See other available formats)
Regulatory Status
RUO
Other Names
FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, glycoprotein IIa, integrin VLA-4 beta subunit, integrin beta-1, very late activation protein, beta polypeptide
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P5D2_PURE_CD29_Antibody_020317
Human peripheral blood lymphocytes were stained with purified anti-CD29 (clone P5D2, filled histogram) antibody or purified mouse IgG1, κ isotype control (open histogram). A goat anti-mouse PE conjugate was used as a secondary antibody.
  • P5D2_PURE_CD29_Antibody_020317
    Human peripheral blood lymphocytes were stained with purified anti-CD29 (clone P5D2, filled histogram) antibody or purified mouse IgG1, κ isotype control (open histogram). A goat anti-mouse PE conjugate was used as a secondary antibody.
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921303 25 µg 89 CHF
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921304 100 µg 218 CHF
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Description

CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P5D2 monoclonal antibody was generated against human fibroblast cells (HT1080).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone P5D2 interferes with binding of integrin-bearing cells to fibronectin, laminin and collagen but not vitronectin coated surfaces.

P5D2 reacts with the integrin β1 subunit and inhibits the function of all β1 integrins. 

Application References

(PubMed link indicates BioLegend citation)
  1. Wayner EA, Hoffstrom BG. 2007. Meths Enzymol In: Integrins. 426:117. PubMed
  2. Wang HQ, et al. 2007. Eur J Cell Biol. 86(1):51. (Block) PubMed
  3. Da Silva MS, et al. 2003. Surgery. 134(2):164. (Block) PubMed
  4. Blaschke R, et al. 2002. Biochem Biophys Res Commun. 296(4):890. (Block) PubMed
  5. Dittel BN, et al. 1993. Blood. 81:2272. (FC) PubMed
Product Citations
  1. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  2. Kim SHJ, et al. 2021. Mol Biol Cell. 32:1749. PubMed
  3. Watanabe K, et al. 2018. Am J Physiol Cell Physiol. 315:C398. PubMed
  4. Lu H, et al. 2020. Am J Reprod Immunol. 83:. PubMed
RRID
AB_2650620 (BioLegend Cat. No. 921303)
AB_2650620 (BioLegend Cat. No. 921304)

Antigen Details

Structure
CD29 is a 130 kD single chain type I glycoprotein.
Distribution
Tissue distribution: Leukocytes, endothelial cells, smooth muscle, epithelial cells, central nervous system, and stem cells.
Cellular distribution: endosome, extracellular, plasma membrane, and cytoskeleton.
Function
Cell-cell and cell-matrix interactions
Ligand/Receptor
VCAM-1, MAdCAM-1, extracellular matrix (ECM)
Cell Type
Leukocytes, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Signal Transduction, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Long K, et al. 2016. Nat. Commun. 7:10354. PubMed 
2. Campos LS. 2005. Bioessays. 27(7):698.

Gene ID
3688 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org
Go To Top Version: 0    Revision Date: 02.13.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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