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Purified anti-CX3CL1 (Fractalkine) Antibody

Pricing & Availability
Clone
L393H11 (See other available formats)
Regulatory Status
RUO
Other Names
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1), ABCD-3, Fractalkine, Neurotactin (NTT, NTN), Small Inducible Cytokine Subfamily D (Cys-X3-Cys), Member-1 (SCYD1), CXC3C
Isotype
Mouse IgG2b, κ
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Product Citations
publications
L393H11_CX3CL1_Human_Purified_WB_072314.jpg
Western blot analysis of 50 ng recombinant mouse CX3CL1 (lane 1) and human CX3CL1 (lane 2) using anti-human CX3CL1 antibody (clone L393H11).
  • L393H11_CX3CL1_Human_Purified_WB_072314.jpg
    Western blot analysis of 50 ng recombinant mouse CX3CL1 (lane 1) and human CX3CL1 (lane 2) using anti-human CX3CL1 antibody (clone L393H11).
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661201 50 µg 112 CHF
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661202 500 µg 335 CHF
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Description

CX3CL1 is the only member of the CX3C chemokine family that is constitutively expressed in endothelial and epithelial cells, lymphocytes, neurons and microglial osteoblasts. CX3CL1 exists in two forms: as a membrane-anchored form of approximately 100 kD or as a shed 80-95 kD glycoprotein. The chemokine domain is roughly 8 kD, but it migrates at about 13 kD. Its membrane bound form promotes the firm adhesion of rolling leucocytes onto the vessel wall, while its soluble form serves as a potent chemoattractant for CX3CR1-expressing cells such as NK cells, CD14+ monocytes, cytotoxic effector T cells, B cells, neurons, microglia, smooth muscle cells, and tumor cells. The CX3CL1/CX3CR1 axis plays an important role in the pathogenesis of several inflammatory diseases such as allergic pathologies, rheumatoid arthritis, atherosclerosis and tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CX3CL1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2563890 (BioLegend Cat. No. 661201)
AB_2563890 (BioLegend Cat. No. 661202)

Antigen Details

Structure
Chemokine, membrane bound form includes an extracellular NH2-terminal domain, a mucin-like stalk, a transmembrane α helix, and a short cytoplasmic tail. Full-length CX3CL1 contains over 300 amino acids and is highly glycosylated.
Distribution

Cell membrane, secreted.

Function
CX3CL1 has potent chemoattractant activity for T cells, monocytes, and natural killer cells, and the expression of CX3CL1 in activated endothelial cells promotes strong adhesion of those leukocytes. CXC3L1 is induced in endothelial cells by proinflammatory cytokines (TNF-α, IFN-γ, IL-1β) and LPS.
Interaction
Monocytic cells, smooth muscle cells, T cells, B cells, NK cells, microglia, and astrocytes.
Ligand/Receptor
CX3CR1
Cell Type
B cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Ferretti E, et al. 2014. Mediators Inflamm. 2014:480941.
2. Apostolakis S, et al. 2013. Acta Pharmacol Sin. 34:1251.
3. Julia V, et al. 2012. Allergy. 67:1106.
4. Bazan JF, et al. 1997. Nature 6617:640.
5. Hundhausen C, et al. 2003. Blood 102:1186.
6. Sato E, et al. 2006. Ann. Rheum. Dis. 65:1257.
7. Jones B, et al. 2011. Front. Immunol. 2:82.

Gene ID
6376 View all products for this Gene ID
UniProt
View information about CX3CL1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.28.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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