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Purified anti-Cytokeratin 18 Antibody

Pricing & Availability
Clone
DA-7 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin 18, Keratin type I cytoskeletal 18
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DA-7_pure_cytokeratin18_Antibody_1_062518
Whole cell lysates (15 µg protein) from A549, A431, HeLa, HEL (negative control) and U87-MG (negative control) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-Cytokeratin 18 antibody, clone DA-7. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Cell lysates were loaded in order of descending KRT18 mRNA expression levels (source: Human Protein Atlas). Lane M: Molecular Weight Marker.
  • DA-7_pure_cytokeratin18_Antibody_1_062518
    Whole cell lysates (15 µg protein) from A549, A431, HeLa, HEL (negative control) and U87-MG (negative control) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-Cytokeratin 18 antibody, clone DA-7. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Cell lysates were loaded in order of descending KRT18 mRNA expression levels (source: Human Protein Atlas). Lane M: Molecular Weight Marker.
  • DA-7_pure_cytokeratin18_Antibody_2_062518
    MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-cytokeratin 18 antibody (clone DA-7). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • DA-7_pure_cytokeratin18_Antibody_3_062518
    MCF-7 cells were stained with anti-Cytokeratin 18 (clone DA-7), followed by Alexa Fluor® 546 secondary antibody and DAPI (nuclei). Images were aquired on a Nikon FC300 inverted microscope at 20X magnification. Data provided by Dr. John Nolan, La Jolla Bioengineering Institute.
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628401 25 µg 101 CHF
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628402 100 µg 218 CHF
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Description

Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein with a molecular weight of approximately 48 kD. Cytokeratin 18 is a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8 and is widely expressed in simple epithelial tissues in adults, where it is found in the cytoplasm, nucleus, and nucleolus. Cytokeratin 18 is associated with the cytoskeleton and mutations in this protein have been associated with liver disease after environmental insult. Cytokeratin 18 can be modified by glycosylation, phosphorylation (Ser34 and Ser53), acetylation (Ser2), and proteolytic cleavage at amino acids 238 and 397 by caspase family members. The cytokeratin 18 protein interacts with a number of proteins including caspase 3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, and usherin, among others.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human breast carcinoma cell line PMC-42
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
ELISA, IHC-P, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 4.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The DA-7 monoclonal antibody recognizes human cytokeratin 18 and is useful for Western blotting. Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistry of paraffin-embedded sections, and ELISA.

This clone and BioLegend clone LDK18 displayed similar affinities when tested for western blot applications

Application References

(PubMed link indicates BioLegend citation)
  1. Taylor-Papadimitriou J, et al. 1989. J. Cell Sci. 94:403.
  2. Lauerova L, et al. 1988. Hybridoma 7:495.
Product Citations
  1. Valentine H, et al. 2022. Cancer Res Commun. 2:1355. PubMed
  2. Urano-Takaoka M, et al. 2022. Acta Derm Venereol. 102:adv00636. PubMed
RRID
AB_439771 (BioLegend Cat. No. 628401)
AB_439771 (BioLegend Cat. No. 628402)

Antigen Details

Structure
Type I intermediate filament protein, contains three coiled-coil domains, approximately 48 kD. Heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8.
Distribution

Widely expressed in simple epithelial tissues in adults. Can be found in the cytoplasm, nucleus, and nucleolus

Function
Intermediate filament protein involved with the cytoskeleton. Mutations in cytokeratin 18 may predispose to liver disease upon exposure to some viruses or hepatoxins
Interaction
Interacts with a number of proteins including caspase3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, usherin
Modification
Phosphorylation (Ser34, Ser53), glycosylation, acetylation (Ser2). Can also be cleaved at amino acid 238 and 397 by caspase family members
Cell Type
Epithelial cells
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Ku N, et al. 2003. Proc. Natl. Acad. Sci. 100:6063.
2. Kulesh DA and Oshima RG. 1989. Genomics 4:339.
3. Steinart PM and Roop DR. 1988. Ann. Rev. Biochem. 57:593.

Gene ID
3875 View all products for this Gene ID
UniProt
View information about Cytokeratin 18 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06.25.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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