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Purified anti-HA.11 Epitope Tag Antibody (Previously Covance catalog# MMS-101P)

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# MMS-101P
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_16B12_Purified_HA.11_Antibody_1_WB_061915
Western blot of Clone 16B12. Lane 1: Molecular weight marker; Lane 2: Posi-tag control Protein (Cat # 931301); Lane 3: HA.11 overexpressing cell lysate; Lane 4: Untransfected cell lysate.
  • 1_16B12_Purified_HA.11_Antibody_1_WB_061915
    Western blot of Clone 16B12. Lane 1: Molecular weight marker; Lane 2: Posi-tag control Protein (Cat # 931301); Lane 3: HA.11 overexpressing cell lysate; Lane 4: Untransfected cell lysate.
  • 2_16B12_PURE_HA-11_EpitopeTag_Antibody_120321
    Transfected RBL1 cells expressing the HA tag on the cell surface were stained with purified anti-HA.11 epitope Tag (clone 16B12) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by APC conjugated goat-anti-mouse IgG.
  • 3_16B12_Purified_HA.11_Antibody_2_ICC_081415
    Staining of Clone 16B12 on methanol fixed CHO cells transfected with an HA-tagged protein at 1 µg/ml. Cells were then incubated with Alexa Fluor® 594 Goat anti-mouse IgG secondary and were counterstained with DAPI (blue).
  • 4_16B12_Purified_HA.11_Antibody_2_WB_102317
    Immunoprecipitation of HA protein from CHO and CHO-HA stable cell extracts using HA Mouse primary antibody. Western blot analysis was performed using Direct-Blot™ HRP anti-HA (#901519). Direct-Blot™ HRP anti-β-actin (#643807) was used as a lysate loading control.
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901533 25 µL 59 CHF
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901501 200 µL 356 CHF
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901502 500 µL 556 CHF
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901503 1 mL 846 CHF
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
Phosphate-buffered solution + 50% Glycerol + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
Store between 2°C and 8°C.
Application

WB - Quality tested
FC, ICC, IP - Verified
Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is a dilution of 1:2,000-1:20,000. For immunocytochemistry, the suggested use of this reagent is a dilution of 1:1,000 is suggested. For immunoprecipitation, the suggested use of this reagent is a dilution of 1:50 - 1:100 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
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RRID
AB_2801249 (BioLegend Cat. No. 901533)
AB_2565006 (BioLegend Cat. No. 901501)
AB_2565007 (BioLegend Cat. No. 901502)
AB_2565005 (BioLegend Cat. No. 901503)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

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Go To Top Version: 10    Revision Date: 12.03.2021

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