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Ultra-LEAF™ Purified anti-HA.11 Epitope Tag Antibody

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_16B12_ULEAF_HAdot11_Antibody_WB_082417
Total cell lysate (15 µg protein) from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (1 µg/mL), 1:4000 diluted (0.25 µg/mL) and 1:10000 diluted (0.1 µg/mL) purified anti-HA.11 Epitope Tag antibody (clone 16B12) (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG antibody. 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower).
  • 1_16B12_ULEAF_HAdot11_Antibody_WB_082417
    Total cell lysate (15 µg protein) from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (1 µg/mL), 1:4000 diluted (0.25 µg/mL) and 1:10000 diluted (0.1 µg/mL) purified anti-HA.11 Epitope Tag antibody (clone 16B12) (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG antibody. 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower).
  • 2_16B12_PURE_HA-11_EpitopeTag_Antibody_120321.png
    Transfected RBL1 cells expressing the HA tag on the cell surface were stained with purified anti-HA.11 epitope Tag (clone 16B12) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by APC conjugated goat-anti-mouse IgG.
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901521 100 µg 270 CHF
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901522 1 mg 715 CHF
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
FC - Verified
Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:2000-1:20000 diluted (0.05 - 0.5 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
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  4. Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
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Product Citations
  1. Todd NK, et al. 2022. Cell Rep. 40:111110. PubMed
  2. Seth A, et al. 2023. J Exp Med. :220. PubMed
  3. Hafer TL, et al. 2023. Viruses. :15. PubMed
  4. Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed
RRID
AB_2716059 (BioLegend Cat. No. 901521)
AB_2716059 (BioLegend Cat. No. 901522)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 3    Revision Date: 12.03.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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