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Purified anti-Histone H3 Phospho (Ser10) Antibody

Pricing & Availability
Clone
11D8 (See other available formats)
Regulatory Status
RUO
Other Names
Histone-H3, H3
Isotype
Mouse IgG2b, κ
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Product Citations
publications
11D8_061711
293T cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-H3-pS10 clone 11D8 antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1, is inter phase 293T cell extract; lane 2, is M phase 293T cell extract (overnight nocodazole-treated cells).
  • 11D8_061711
    293T cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-H3-pS10 clone 11D8 antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1, is inter phase 293T cell extract; lane 2, is M phase 293T cell extract (overnight nocodazole-treated cells).
  • 11D8_1_071511
  • 11D8_2_071511
    Exponentially growing Hela cells were stained with purified phospor-Histone H3 (Ser10), clone 11D8 antibody, followed by Alexa Fluor® 488 conjugated to anti-mouse IgG and DAPI. The phosphor-Histone staining is specific on metaphase cells only.
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650801 25 µg 176 CHF
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650802 100 µg 411 CHF
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Description

Histone H3 is phosphorylated at serine 10 during mitosis and is found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified synthetic peptide conjugated to KLH
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IF - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting.

Product Citations
  1. Friedman J, et al. 2018. J Immunother Cancer. 6:59. PubMed
  2. Han G, et al. 2018. Nat Protoc. 2.014583333. PubMed
RRID
AB_10896911 (BioLegend Cat. No. 650801)
AB_10896911 (BioLegend Cat. No. 650802)

Antigen Details

Structure
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution

Nucleus

Function
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability.
Interaction
Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.
4. Hans F, et al. 2001. Oncogene 20:3021.

Regulation
H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Gene ID
8290 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org
Go To Top Version: 3    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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