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Purified anti-human CD11c Antibody

Pricing & Availability
Clone
3.9 (See other available formats)
Regulatory Status
RUO
Workshop
III NL707
Other Names
Integrin αX subunit, CR4, p150, ITGAX
Isotype
Mouse IgG1, κ
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Product Citations
publications
3.9_Pure_052412
Human peripheral blood granulocytes were stained with purified CD11c (clone 3.9) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • 3.9_Pure_052412
    Human peripheral blood granulocytes were stained with purified CD11c (clone 3.9) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
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301601 25 µg 59 CHF
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301602 100 µg 105 CHF
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Description

CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αX and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
  3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
  4. Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC)
  5. Ottonello L, et al. 1999. Blood 93:3505.
  6. Metelitsa LS, et al. 2002. Blood 99:4166.
  7. Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed
  8. Ihanus E, et al. 2007. Blood 109:802-810.
  9. Gurer C, et al. 2008. Blood 112:1231. PubMed
  10. Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed
  11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  12. Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC)
Product Citations
  1. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  2. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  3. Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed
  4. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  5. Gurer C, et al. 2008. Blood. 112:1231. PubMed
  6. Santos R, et al. 2017. Nat Commun. . 10.1038/s41467-017-01760-5. PubMed
  7. Sadhu C, et al. 2007. J Leukoc Biol . 81:1395. PubMed
  8. Kremenovic M, et al. 2020. Cancers (Basel). 12:. PubMed
  9. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  10. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  11. Gonzalez VD, et al. 2021. Cell Reports. 36(9):109632. PubMed
  12. Liu X, et al. 2022. STAR Protoc. 3:101310. PubMed
  13. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  14. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  15. Lukácsi S, et al. 2020. PLoS One. 15:e0232432. PubMed
  16. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  17. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  18. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  19. Asai A, et al. 2009. J Lipid Res. 83:4365. PubMed
  20. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  21. Delgado-Gonzalez A, et al. 2022. STAR Protoc. 3:101425. PubMed
  22. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  23. Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID
AB_314171 (BioLegend Cat. No. 301601)
AB_314171 (BioLegend Cat. No. 301602)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Distribution

Myeloid, dendritic cells, NK cells, B cells and T cell subsets

Function
Adhesion, CTL killing
Ligand/Receptor
CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
Cell Type
B cells, Dendritic cells, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Petty H. 1996. Immunol. Today 17:209.
2. Springer T. 1994. Cell 76:301.
3. Ihanus E, et al. 2007. Blood 109:802-810.

Gene ID
3687 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org
Go To Top Version: 2    Revision Date: 08.18.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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