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Purified anti-human CD202b (Tie2/Tek) Antibody

Pricing & Availability
Clone
A19069E (See other available formats)
Regulatory Status
RUO
Other Names
Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2 (hTIE2), p140 TEK, CD202
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A. A19069E_PURE_CD202b_FC_092624
Human endothelial cell line, HUVEC, was stained with purified anti-human CD202b (Tie2/Tek) (clone A19069E) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram) followed by ant-mouse IgG PE.
  • A. A19069E_PURE_CD202b_FC_092624
    Human endothelial cell line, HUVEC, was stained with purified anti-human CD202b (Tie2/Tek) (clone A19069E) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram) followed by ant-mouse IgG PE.
  • B. A19069E_PURE_CD202b_Direct-ELISA_092624
    Direct ELISA showing purified anti-human TIE2 (clone A19069E) bound to plate-immobilized recombinant human Tie-2-Fc chimera (carrier-free) (Cat. No. 784306). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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390552 100 µg 113 CHF
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Description

CD202b, also known as Tie2 or TEK, is a 145 kD type I transmembrane protein. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b that promotes, maintains, and stabilizes mature vessels and maintains HSCs in a quiescent state. Ang-2, another ligand of CD202b, is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant extracellular domain of human Tie2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Each lot of this antibody is verified by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
Clone A19069E partially blocks the staining of reference clone 33.1

Recombinant protein completely blocks the staining of test clone A19069E.

Antigen Details

Structure
145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
Distribution

Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes

Function
Angiogenesis, hematopoiesis, monocytes chemotaxis
Interaction
Endothelial cells, hematopoietic stem and progenitors, monocytes
Ligand/Receptor
Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
Cell Type
Endothelial cells, Hematopoietic stem and progenitors, Monocytes
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. De Palma M, et al. 2005. Cancer Cell. 8::211-26.
  2. Shaw JP, et al. 2004. Blood Cells Mol Dis. 32:168-75.
  3. Hsu HC, et al. 2000. Blood. 96:3757-62.
  4. Arai F, et al. 2004. Cell. 118:149-61.
Gene ID
7010 View all products for this Gene ID
UniProt
View information about CD202b on UniProt.org
Go To Top Version: 1    Revision Date: 09.26.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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