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Purified anti-human CD243 (ABCB1) Antibody

Pricing & Availability
Clone
4E3.16 (See other available formats)
Regulatory Status
RUO
Other Names
MDR-1, Multidrug resistance protein 1, P-glycoprotein 1, colchicin sensitivity, doxorubicin resistance
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
4E3point16_Pure_CD243_ABCB1_Antibody_010816
Human neuroblastoma cell line, SK-N-FI, was stained with purified human CD243 (clone 4E3.16) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 4E3point16_Pure_CD243_ABCB1_Antibody_010816
    Human neuroblastoma cell line, SK-N-FI, was stained with purified human CD243 (clone 4E3.16) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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919403 100 µg 288 CHF
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Description

CD243 (MDR-1) belongs to the ATP binding cassette (ABC) transporter family. With an approximate molecular mass of 170 kD, it consists of two homologous halves. Each half contains two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs). The TMDs span the membrane six times, forming a chamber with a 12 transmembrane α-helix structure. NBDs drive the transport process through ATP coupling and hydrolysis and are located at the cytoplasmic face of the membrane. CD243 transports various molecules across cellular membranes and is involved in multidrug resistance. MDR-1 is expressed on hematopoietic stem cells, T cells, B cells, and NK cells as well as on many multidrug resistant neoplastic cells. CD243 interacts with Caveolin, RING finger protein 1B, AAP1, p53, Orphan nuclear receptor PX, and cytochrome P450.

 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SW-1573 cells selected by resistance on doxorubicin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Arceci RJ, et al. 1993. Cancer Res. 53:310.
  2. Arceci RJ, et al. 1992. Blood 80:1528-36.
  3. Schinkel AH, et al. 1993. Int. J. Cancer 55:478.
  4. Huet S, et al. 1998. Cytometry 34(6):248-56.
  5. Müller MR, et al. 2000. Int. J. Clin. Pharmacol. Ther. 38:180-6.
  6. Den Boer ML, et al. 1997. Leukemia 11:1078-85.
Product Citations
  1. Simonneau C, et al. 2021. Fluids Barriers CNS. 18:43. PubMed
RRID
AB_2571901 (BioLegend Cat. No. 919403)

Antigen Details

Structure
Member of ABC transporter family with an approximate molecular weight of 170 kD. Consists of two homologous halves, each comprising two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs).
Distribution

Hematopoietic stem cells, T and B cells, natural killer (NK) cells, and many multidrug resistant neoplastic cells.

Function
Transports various molecules across cellular membranes and is involved in multidrug resistance.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Dulucq S, et al. 2008. Blood 112:2024.
  2. Gottesman MM, et al. 2009. Nat. Biotechnol. 27:546.
  3. Andreadis C, et al. 2007. Blood 109:3409.
Gene ID
5243 View all products for this Gene ID
UniProt
View information about CD243 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03.18.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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