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Purified anti-human CD283 (TLR3) Antibody

Pricing & Availability
Clone
TLR-104 (See other available formats)
Regulatory Status
RUO
Other Names
Toll like receptor 3, TLR-3, CD283
Isotype
Mouse IgG2a, κ
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Product Citations
publications
TLR-104_CD283_Purified_Antibody_WB_012114.jpg
Western blot analysis of extracts from 293T parent (lane 1) and 293T transfected with human TLR3 (lane 2) using anti-CD283 antibody(TLR-104).
  • TLR-104_CD283_Purified_Antibody_WB_012114.jpg
    Western blot analysis of extracts from 293T parent (lane 1) and 293T transfected with human TLR3 (lane 2) using anti-CD283 antibody(TLR-104).
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315002 100 µg 293 CHF
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Description

Toll-like receptor 3 (TLR3) is a 104 kD protein also known as CD283. It is expressed selectively on the cytoplasmic membrane and intracellularly in dendritic cells. TLR3 is also highly expressed in the placenta, pancreas, heart, liver, lung, and muscle. TLR3 is a pattern recognition receptor that participates in innate immune response to microbial pathogens. The ligands recognized by TLR3 are polyinosine-polycytidylic acid (Poly(I:C)) and dsRNA. TLR3 ligands induce NF-κB activation (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 has been shown to interact with a number of proteins including MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2. This antibody is useful for flow cytometry and Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Toll-like receptor 3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC, WB - Quality tested
 
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. For Western blotting, suggested working dilution(s)is 10 µg antibody in 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western bloting. The fluorochrome-labeled TLR-104 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis. For intracellular staining protocol, please visit www.biolegend.com and click on the support tab.

Application References

(PubMed link indicates BioLegend citation)
  1. Fiala M, et al. 2007. P. Natl. Acad. Sci. USA 10.1073/P. Natl. Acad. Sci. USA.0701267104.
  2. Kong KF, et al. 2008. J. Virol. 82:7613. PubMed
  3. Sadik CD, et al. 2009. Nucleic Acids Res. 37:5041. PubMed
Product Citations
  1. Kong K, et al. 2008. J Virol. 82:7613. PubMed
  2. Sadik C, et al. 2009. Nucleic Acids Res. 37:5041. PubMed
RRID
AB_2303470 (BioLegend Cat. No. 315002)

Antigen Details

Structure
Toll-like receptor family member, type I membrane protein containing multiple leucine repeats and a interleukin-1 receptor like region in the extracellular domain, predicted molecular weight approximately 104 kD
Distribution

Selective expression in dendritic cells. Highly expressed in placenta, pancreas, also expressed in heart, liver, lung and muscle

Function
Pattern recognition receptor that participates in innate immune response to microbial pathogens. TLR3 ligands induce NF-κB (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 stimulation results in the production of the cytokines
Interaction
MYD88, TRAF6, TRIAD3, MAP3K7, TAB2
Ligand/Receptor
Polyinosine-polycytidylic acid (Poly(I:C)), dsRNA
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Alexopoulou L, et al. 2001. Nature 413:732.
2. Doyle SE, et al. 2002. Immunity 17:251.
3. Meylan E, et al. 2004. Nature Immun. 5:503.
4. Muzio M, et al. 2000. J. Immunol. 164:5998.
5. Rock FL, et al. 1998. Proc. Natl. Acad. Sci. 95:588.

Gene ID
7098 View all products for this Gene ID
UniProt
View information about CD283 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01.21.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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