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Purified anti-MyD88 Antibody

Pricing & Availability
Clone
O91B8 (See other available formats)
Regulatory Status
RUO
Other Names
Myeloid Differentiation Primary Response Protein MyD88, MYD88D
Isotype
Mouse IgG1, κ
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Product Citations
publications
O91B8_Purified_MyD88_Antibody_WB_081516
Total cell lysate from 293E cells (lane 1, 15 µ), Jurkat cells (lane 2, 15 µg) and Raw264.7 (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-MyD88 antibody (clone O91B8). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • O91B8_Purified_MyD88_Antibody_WB_081516
    Total cell lysate from 293E cells (lane 1, 15 µ), Jurkat cells (lane 2, 15 µg) and Raw264.7 (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-MyD88 antibody (clone O91B8). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
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675802 100 µg 229 CHF
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Description

MyD88, originally isolated as myeloid differentiation primary response gene, is expressed in a variety of tissues.  MyD88 serves as a cytoplasmic adaptor protein involved in the signaling of TLR and IL-1R family members.  It associates with and recruits IRAK to IL-1R complex in response to IL-1. This pathway leads to activation of NF-kB. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18. MyD88 deficient mice lack a response to LPS (a bacterial product) which employs toll like receptors 2 and 4 (TLR2 and TLR4) as signaling receptors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant MyD88 protein produced in 293T cells.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
RRID
AB_2565607 (BioLegend Cat. No. 675802)

Antigen Details

Structure
A 296 amino acid protein encoded by 5 exons. MW around 33 kD.
Distribution

Expressed by various tissues including lymphocytes, monocytes, heart, liver and kidney.

Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response.
Interaction
TIRAP, TLR2, TLR4, IRAK1, IRAK2, IL18R1, IRF-1 et al.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Bonnert TP, et al. 1997. FEBS Letters 402:81.
2. Burns K, et al. 1998. J. Biol. Chem. 20:12203.
3. Ohnishi H, et al. 2009. Proc. Natl. Acad. Sci. USA 106:10260.

Gene ID
4615 View all products for this Gene ID
UniProt
View information about MyD88 on UniProt.org
Go To Top Version: 3    Revision Date: 06.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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