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Purified anti-human CD324 (E-Cadherin) Antibody

Pricing & Availability
Clone
67A4 (See other available formats)
Regulatory Status
RUO
Other Names
E-Cadherin, cadherin-1, and UVO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
67A4_Purified_CD324_Antibody_1_121817
Human colon carcinoma cell line (HT29) stained with purified CD324 (clone 67A4) (filled histogram), or purified mouse IgG1, κ (open histogram) followed by anti-mouse IgG FITC.
  • 67A4_Purified_CD324_Antibody_1_121817
    Human colon carcinoma cell line (HT29) stained with purified CD324 (clone 67A4) (filled histogram), or purified mouse IgG1, κ (open histogram) followed by anti-mouse IgG FITC.
  • 67A4_Purified_CD324_Antibody_2_121817
    BT474 breast cancer cells were stained with anti-human CD324 (clone 67A4) using 1:100 dilution, followed by DyLight™ 649 anti-mouse Ig secondary antibody (red) plus DAPI staining for nuclei (blue). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, blocked with 10% serum, and incubated O/N at 4°C. Images were taken under 20x (filter set: EX 647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by John Nolan and Er Liu, La Jolla Bioengineering Institute.
  • 67A4_Purified_CD324_Antibody_3_121817
    Total cell lysate (15 µg) from HeLa (negative control, lane 1), MCF-7 (lane2) and NIH3T3 (lane3) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with 1:500 (1 µg/mL), 1:2000 (0.25 µg/mL) and 1:5000 (0.1 µg/mL) diluted purified anti-human CD324 (E-Cadherin) Antibody (clone 67A4). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (Cat No. 643807) was used as a loading controls (lower). Lane M is the MW ladder.
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324101 25 µg 101 CHF
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324102 100 µg 206 CHF
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Description

The 67A4 antibody recognizes human CD324 also known as E-cadherin, cadherin-1, and UVO. CD324, a member of the cadherin superfamily, is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region with a predicted molecular weight of approximately 100 kD. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase 8, β-catenin, presenilin 1, casein kinase II , as well as other extracellular proteins including the EGF receptor. CD324 is phosphorylated on multiple residues (S857, S866, S870, S872), and can be proteolytically cleaved at reside D769 by caspase-3. The 67A4 antibody has been shown to be useful for flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
T-47D cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (dilution 1:5000 - 1:500). It is recommended that the reagent be titrated for optimal performance for each application.

It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. 

Application References

(PubMed link indicates BioLegend citation)
  1. Armeanu S, et al. 1995. J. Cell Biol. 131:243.
  2. Bühring HJ, et al. 1996. Leukemia 10:106.
  3. Yauch RL, et al. 2005. Clin. Cancer Res. 11:8686. (WB)
  4. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  5. Ardehali R, et al. 2013. PNAS. 110:3405. PubMed
  6. Rasanen K, et al. 2013. Mol Cell Protecomics. 12:3778. PubMed
  7. Chaudhury A, et al. 2014. Nucleic Acids Res. 42:86. PubMed
  8. Milne P, et al. 2015. Blood. 125:470. PubMed
Product Citations
  1. Valencia J, et al. 2011. J Immunol. 187:4129. PubMed
  2. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  3. Tanaka HY, et al. 2020. Biomaterials. 251:120077. PubMed
  4. Zhuang J, et al. 2019. Adv Sci (Weinh). 6:1901462. PubMed
  5. Kumar M, et al. 2020. Oncotarget. 11:1603. PubMed
  6. Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
RRID
AB_756063 (BioLegend Cat. No. 324101)
AB_756063 (BioLegend Cat. No. 324102)

Antigen Details

Structure
Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region. Predicted molecular weight approximately 100 kD.
Distribution

Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells

Function
Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cell, while the cytoplasmic domain is required for internalization.
Interaction
Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase 8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others
Ligand/Receptor
αEβ7 integrin
Modification
Phosphorylated on multiple residues (S857, S866, S870, S872), proteolytically cleaved at reside D769 by caspase-3.
Cell Type
Embryonic Stem Cells, Epithelial cells, Erythrocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1303.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190.

Gene ID
999 View all products for this Gene ID
UniProt
View information about CD324 on UniProt.org
Go To Top Version: 3    Revision Date: 08.08.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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