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Purified anti-human CD41/CD61 Antibody

Pricing & Availability
Clone
PAC-1 (See other available formats)
Regulatory Status
RUO
Other Names
gpIIb/IIIa complex, integrin αIIbβ3
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
PAC-1_Purified_glycoproteinIIb-IIIa_Antibody_FC_062014.jpg
ADP-activated human platelets (filled histogram) or resting platelets (open histogram) were stained with purified anti-human glycoprotein IIb/IIIa complex (clone PAC-1), followed by anti-mouse IgM FITC.
  • PAC-1_Purified_glycoproteinIIb-IIIa_Antibody_FC_062014.jpg
    ADP-activated human platelets (filled histogram) or resting platelets (open histogram) were stained with purified anti-human glycoprotein IIb/IIIa complex (clone PAC-1), followed by anti-mouse IgM FITC.
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362802 100 µg 229 CHF
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Description

The gpIIb/IIIa complex, also known as integrin αIIbβ3, is expressed by megakaryocytes and platelets. It undergoes conformational changes upon activation by substances, such as ADP, thrombin, and TXA2, which subsequently reveals a ligand binding site that is specific for fibrinogen, von Willebrand factor (vWF), fibronectin, and vitronectin. This conformational change is crucial for platelet-platelet adhesion. Ligand bound gpIIb/IIIa complex promotes platelet aggregation. This complex plays an important role in hemostasis and is a target for developing therapeutic antibodies in immune thrombocytopenia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation1,2, Western blotting2 and in vitro blocking of platelet aggregation2.

 

The PAC-1 antibody reacts with activation-induced conformational epitope of the integrin aIIbß3. PAC-1 binding to fibrinogen is pH and Ca2+ dependent, and will not bind to platelets that have been fixed.  We recommend staining in buffers free of Ethylenediaminetetraacetic acid (EDTA) at a pH of 6.5 or higher.  Sodium citrate may be used as an anticoagulant for blood collection3.

Application References

(PubMed link indicates BioLegend citation)
  1. Shattil SJ, et al. 1987. Blood. 70:307. (IP)
  2. Shattil SJ, et al. 1985. J. Biol. Chem. 260:11107. (Block, IP, WB)
  3. Shattil SJ, et al. 1986. Blood. 68: 1224-31.
Product Citations
  1. Zhang L, et al. 2022. Acta Pharm Sin B. 12:3427. PubMed
  2. Srinivasan S, et al. 2015. J Biol Chem. 290: 25129 - 25139. PubMed
  3. Deng W, et al. 2016. Nat Commun. 7: 12863. PubMed
RRID
AB_2563889 (BioLegend Cat. No. 362802)

Antigen Details

Structure
α/β transmembrane heterodimer of the ubiquitous integrin superfamily
Distribution

Resting platelets; activated platelets express a conformationally modified complex that can bind fibrin

Function
Fibrinogen binding, platelet aggregation
Ligand/Receptor
Fibrinogen
Cell Type
Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hynes RO. 1992. Cell 69:11.
2. Shattil SJ, et al. 1985. J. Biol. Chem. 260:11107.
3. Ginsberg, et al. 1990. Blood 76:2017.
4. O’Toole TE, et al. 1991. Science 254:845.
5. Peterson JA, et al. 1998. Blood 92: 2053.

Gene ID
3674 View all products for this Gene ID 3690 View all products for this Gene ID
UniProt
View information about CD41/CD61 on UniProt.org
Go To Top Version: 2    Revision Date: 03.27.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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