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Purified anti-human CD49b Antibody

Pricing & Availability
Clone
P1E6-C5 (See other available formats)
Regulatory Status
RUO
Other Names
α2 integrin, VLA-2 α chain, Integrin α2 chain
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1E6-C5_Purified_CD49b_Antibody_FC_102813
Human peripheral blood platelets stained with purified CD49b (clone P1E6-C5) or purified mouse IgG1, κ isotype control, followed by anti-mouse IgG PE.
  • P1E6-C5_Purified_CD49b_Antibody_FC_102813
    Human peripheral blood platelets stained with purified CD49b (clone P1E6-C5) or purified mouse IgG1, κ isotype control, followed by anti-mouse IgG PE.
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359301 50 µg 101 CHF
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359302 500 µg 382 CHF
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Description

CD49b is a 170 kD transmembrane protein, also known as α2 integrin, VLA-2 α chain, Integrin α2 and GPIa. It associates with CD29 (β1 integrin) to form VLA-2, a collagen and laminin receptor on many cell types including monocytes, platelets, activated T cells, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts. CD49b has been reported to interact with F-actin and matrix metalloproteinase 1. CD49b is a platelet alloantigen and has been associated with neonatal alloimmune thrombocytopenia. Deficiencies in this protein have been associated with hemorrhagic disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HT1080 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: in vitro blocking activity1,2, immunoprecipitation3, and ELISA4. 

Application References

(PubMed link indicates BioLegend citation)
  1. Hirsch MS, et al. 1997. Dev. Dyn. 210:249. (Block)
  2. Sawhney RS, et al. 2006. J. Biol. Chem. 281:8497. (Block)
  3. Lee SA, et al. 2009. Carcinogensis. 30:1872. (IP)
  4. Zßrate S, et al. 2004. J. Virol. 78:10839. (ELISA)
Product Citations
  1. van der Sluis TC, et al. 2023. Cell Rep Med. 100939:4. PubMed
  2. Watanabe K, et al. 2018. Am J Physiol Cell Physiol. 315:C398. PubMed
RRID
AB_2562681 (BioLegend Cat. No. 359301)
AB_2562681 (BioLegend Cat. No. 359302)

Antigen Details

Structure
Integrin alpha chain family, type I membrane protein, alpha chain of heterodimeric integrin receptor, 170 kD; associates with CD29 to form collagen, laminin receptor
Distribution

Broadly expressed on many cell types including B cells, monocytes, platelets, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts

Function
With CD29; binds collagen and laminin
Ligand/Receptor
Forms heterodimer with integrin β1 (CD29) which is a receptor for laminin and collagen
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kaplan C, et al. 1991. Br. J. Haematol. 78:425.
2. Kiefel V, et al. 1991. Vox Sang. 60:244.
3. Nieuwenhuis HK, et al. 1985. Nature 318:470.
4. Takada Y and Helmer ME. 1989. J. Cell Biol. 109:397.

Gene ID
3673 View all products for this Gene ID
UniProt
View information about CD49b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11.25.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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