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Purified anti-human CD49e Antibody

Pricing & Availability
Clone
NKI-SAM-1 (See other available formats)
Regulatory Status
RUO
Other Names
α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
NKI-SAM1_Pure_122007
Human peripheral blood lymphocytes stained with purified NKI-SAM1, followed by anti-mouse IgG FITC
  • NKI-SAM1_Pure_122007
    Human peripheral blood lymphocytes stained with purified NKI-SAM1, followed by anti-mouse IgG FITC
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328002 100 µg 118 CHF
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Description

CD49e is a type I integral membrane glycoprotein, known as α5 integrin, VLA-5 α chain. It forms noncovalent heterodimer with integrin β1 (CD29). CD49e contains two disulfide-linked subunits of 135 kD and 24 kD, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
U937 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and inhibition of a5 integrin mediated cell adhesion2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 328004).

Application References

(PubMed link indicates BioLegend citation)
  1. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  2. Orecchia A, et al. 2003. J. Cell Sci. 116:3479. (Block)
Product Citations
  1. Park S, et al. 2019. Sci Rep. 9:13286. PubMed
  2. Sivasubramaniyan K, et al. 2015. Glycobiology. 25: 902-917. PubMed
  3. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  4. Burckhardt CJ, et al. 2021. Developmental Cell. 56(8):1164-1181.e12. PubMed
RRID
AB_893363 (BioLegend Cat. No. 328002)

Antigen Details

Structure
Integrin family, associates with integrin ß1 (CD29), 135 kD and 25 kD in reduced condition, 155 kD (unreduced)
Distribution

Thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, some myeloid leukemia cells

Function
Adhesion, regulation of cell survival and apoptosis
Ligand/Receptor
Fibronectin, neural adhesion molecule L1
Cell Type
Endothelial cells, Leukemia, Lymphocytes, Mesenchymal Stem Cells, Osteoclasts, Thymocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1.Tran Van Nhieu GT and Isberg RR. 1991. J. Biol. Chem. 266:24367.
2. Isaji T, et al. 2006.J. Biol. Chem. 281:33258.

Gene ID
3678 View all products for this Gene ID
UniProt
View information about CD49e on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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