Purified anti-human HLA-G Antibody anti-HLA-G

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Clone: 87G (See other available formats)
Regulatory Status: RUO
Other Names: Human Leukocyte Antigen-G
Isotype: Mouse IgG2a, κ
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Product Citations: publications

Human cytotrophoblastic cell line (JEG-3) stained with purified 87G, followed by anti-mouse IgG FITC

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335902

100 µg

118 CHF

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Description

HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A,B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2(CD85j), ILT4(CD85d), and KIR2DL4(CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in thymus.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HLA-G transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
ELISA, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: 87G mAb reacts with isoforms of HLA-G1 and -G5. Additional reported applications (for the relevant formats) include: restoring HLA-G mediated suppression of allo-T cell proliferation and immunohistochemical staining of frozen tissue sections. Clone 87G is reported not to bind HLA-G in aldehyde fixed samples.⁴
RRID: AB_1227707 (BioLegend Cat. No. 335902)

Antigen Details

Structure: Ig
Distribution: Trophoblast cells, thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, various tumors
Function: Inhibit NK and T cell-mediated cytolysis and T cell proliferation, induce apoptosis of activated CD8⁺ T cells, induce Th2 cytokine production
Ligand/Receptor: ILT2(CD85j), ILT4(CD85d), KIR2DL4(CD158d), CD8α/α
Cell Type: Dendritic cells, Epithelial cells, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: 1. Hunt JS, et al. 2005. FASEB J. 19:681.
2. Carosella ED, et al. 2008. Blood 111:4862.
Gene ID: 3135 View all products for this Gene ID
UniProt: View information about HLA-G on UniProt.org

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Other Formats

View All HLA-G Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human HLA-G	87G	FC,ELISA,IHC-F
PE anti-human HLA-G	87G	FC
APC anti-human HLA-G	87G	FC
PE/Cyanine7 anti-human HLA-G	87G	FC
Alexa Fluor® 488 anti-human HLA-G	87G	FC
PE/Dazzle™ 594 anti-human HLA-G	87G	FC
Ultra-LEAF™ Purified anti-human HLA-G	87G	FC,ELISA,IHC-F

Certificate of Analysis

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Go To Top Version: 4 Revision Date: 08.12.2022

For Research Use Only. Not for diagnostic or therapeutic use.

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