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Purified anti-human Tim-4 Antibody

Pricing & Availability
Clone
9F4 (See other available formats)
Regulatory Status
RUO
Other Names
SMUCKLER (Spleen, mucin-containg, knock-out of lymphotoxin (LT)), TIMD4 (T-cell immunoglobulin and mucin domain containing 4)
Isotype
Mouse IgG1, κ
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Product Citations
publications
9F4_Pure_011712
Human Tim-4 transfected CHO cells were stained with purified Tim-4 (clone 9F4) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 9F4_Pure_011712
    Human Tim-4 transfected CHO cells were stained with purified Tim-4 (clone 9F4) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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354002 100 µg 229 CHF
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Description

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th-1 and Th-2) cell-mediated immunity. Tim-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of Tim-1. This binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, Tim-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The Tim-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and is connected to Tim-1-triggered Th2 responsiveness.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TIM-4- IgV Fc, fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 9F4 recognizes the FG loop of human Tim-41.

Application References

(PubMed link indicates BioLegend citation)
  1. Kobayashi N, et al. 2007. Immunity 27:927.
Product Citations
  1. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
RRID
AB_11126746 (BioLegend Cat. No. 354002)

Antigen Details

Structure
Type 1 transmembrane glycoprotein and mucin domain containing protein 4.
Distribution

Splenic CD14+, macrophages, immature DC.

Function
Tim-1 ligation to Tim-4 induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
Ligand/Receptor
Phosphatiylserine is the receptor of Tim-4, and Tim-4 is a ligand of Tim-1.
Cell Type
Dendritic cells, Macrophages
Biology Area
Immunology
Antigen References

1. Kobayashi N, et al. 2007. Immunity 27:927.
2. Meyers JH, et al. 2005. Nat. Immunol. 6:455.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Shakhov AN, et al. 2004. Eur. J. Immunol. 34:494.

Gene ID
91937 View all products for this Gene ID
UniProt
View information about Tim-4 on UniProt.org
Go To Top Version: 2    Revision Date: 05.15.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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