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Purified anti-Keratin 1 Antibody

Pricing & Availability
Clone
Poly19052 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type II cytoskeletal 1, CK-1, cytokeratin-1, 67 kD cytokeratin, hair alpha protein, type-II keratin Kb1, epidermolytic hyperkeratosis 1, krt1
Isotype
Rabbit Polyclonal
Ave. Rating
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Product Citations
publications
1_Poly19052_PURE_Keratin1_Antibody_3_WB_031519.png
Total cell lysates (15 µg total protein) from A549 and HEL (negative controls) and HaCaT (positive control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-KRT1 Antibody, clone Poly19052, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-β actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted KRT1 expression data obtained from Human Protein Atlas.
  • 1_Poly19052_PURE_Keratin1_Antibody_3_WB_031519.png
    Total cell lysates (15 µg total protein) from A549 and HEL (negative controls) and HaCaT (positive control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-KRT1 Antibody, clone Poly19052, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-β actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted KRT1 expression data obtained from Human Protein Atlas.
  • Poly19052_PURE_Keratin1_Antibody_1_IHCP_062816
    IHC staining of anti-Keratin 1 antibody (Poly19052) on formalin-fixed paraffin-embedded normal human skin. After antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
  • Poly19052_PURE_Keratin1_Antibody_2_WB_062816
    Western blot of anti-Keratin 1 antibody (Poly19052). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 1 µg/ml for 24 hours at 4°C, followed by incubation with a goat anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection.
See high resolution IHC-P data...
Cat # Size Price Quantity Check Availability Save
905203 25 µg 125 CHF
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905204 100 µg 350 CHF
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Description

Keratin 1 is a member of the keratin family of proteins. It is a type II cytokeratin specifically expressed in the spinous and granular layers of the epidermis along with family member Keratin 10. Mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Reported Reactivity
Non-Human Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
The antibody was raised against a peptide sequence derived from the C-terminus of the human keratin 1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
IHC-F, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is suggested. For Western blotting, a concentration range of 0.25 - 1.0 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Wu N, et al. 2012. J. Biol. Chem. 287:5627. (WB) PubMed
  2. Schütz M, et al. 2011. Hum. Mol. Genet. 20:28. (IHC) PubMed
  3. Hu Y, et al. 2001. Nature 410:710. PubMed
  4. Yuspa SH, et al. 1989. J. Cell Biol. 109:1207. PubMed
  5. Roop DR, et al. 1984. J. Biol. Chem. 259:8037. PubMed
Product Citations
  1. Miyauchi K, et al. 2021. Front Immunol. 12:663177. PubMed
  2. Chalmers FE, et al. 2022. Dev Biol. 485:9. PubMed
  3. Noguchi S, et al. 2019. Commun Biol. 2:37. PubMed
  4. Xu M, et al. 2019. Am J Transl Res. 11:257. PubMed
  5. Li J, et al. 2021. Nat Commun. 12:784. PubMed
RRID
AB_2734677 (BioLegend Cat. No. 905203)
AB_2734677 (BioLegend Cat. No. 905204)

Antigen Details

Structure
Keratin 1 is a 66 kD protein.
Distribution

Extracellular, nucleus, and cytoskeleton.

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3848 View all products for this Gene ID
UniProt
View information about Keratin 1 on UniProt.org
Go To Top Version: 3    Revision Date: 08.12.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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